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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KRAS-2A-specific Polyclonal antibody

KRAS-2A-specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 16156-1-AP

Synonyms

KRAS-2A, KRAS, KRAS4A, KRAS4B, pan RAS

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using KRAS-2A-specific Polyclonal antibody (16156-1-AP)

    WB analysis of NIH/3T3 using 16156-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16156-1-AP (KRAS-2A-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KRAS-2A-specific Polyclonal antibody (16156-1-AP)

    WB analysis using 16156-1-AP

    Recombinant proteins (ag27444 (6*His tag-KRAS-2A), ag21645(6*His tag-KRAS-2B), ag29746(6*His tag-NRAS), ag23265(GST-HRAS)) were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using KRAS-2A-specific Polyclonal antibody (16156-1-AP)

    IHC staining of human stomach using 16156-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16156-1-AP (KRAS-2A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KRAS-2A-specific Polyclonal antibody (16156-1-AP)

    IF Staining of HeLa using 16156-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KRAS-2A-specific antibody (16156-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Flow cytometry (FC) experiment of HeLa cells using KRAS-2A-specific Polyclonal antibody (16156-1-AP)

    FC experiment of HeLa using 16156-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KRAS-2A-specific (16156-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

16156-1-AP targets KRAS-2A-specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight 21 kDa
Observed molecular weight 21-24 kDa
GenBank accession numberNM_033360
Gene symbol KRAS
Gene ID (NCBI) 3845
RRIDAB_2234477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAS (derived from 'RAt Sarcoma' virus) gene family includes KRAS, HRAS, and NRAS. These genes encode very similar 21 kDa proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 2A and 2B. This antibody is specific for KRAS-2A, which also named as KRAS-4A .

Protocols

Product Specific Protocols
WB protocol for KRAS-2A-specific antibody 16156-1-APDownload protocol
IHC protocol for KRAS-2A-specific antibody 16156-1-APDownload protocol
IF protocol for KRAS-2A-specific antibody 16156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Chem Biol

Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System.

Authors - Sascha Röth
View Article
humanWB

Sci Signal

Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies.

Authors - Andrew M Waters
View Article
humanWB

Life Sci Alliance

A new ferrocene derivative blocks K-Ras localization and function by oxidative modification at His95

Authors - Kristen M Rehl
View Article