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KRAS-2B Polyclonal antibody
KRAS-2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 16155-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, mouse brain tissue, Recombinant protein |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| FC | See 1 publications below |
Product Information
16155-1-AP targets KRAS-2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | NM_004985 |
| Gene symbol | KRAS |
| Gene ID (NCBI) | 3845 |
| RRID | AB_2134119 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RAS gene family encodes very similar 21KD proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 2A and 2B. This antibody is specific for KRAS-2B.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KRAS-2B antibody 16155-1-AP | Download protocol |
| IHC protocol for KRAS-2B antibody 16155-1-AP | Download protocol |
| IF protocol for KRAS-2B antibody 16155-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors. | ||
Nat Biomed Eng Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting. | ||
Cell Chem Biol Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System. | ||
Sci Signal Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies. | ||
Life Sci Alliance A new ferrocene derivative blocks K-Ras localization and function by oxidative modification at His95 |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Christopher (Verified Customer) (12-14-2018) | Antibody is very specific but also quite weak. Maybe you can increase the primary antibody dilution to 1:500 to increase signal. There is no unspecific recognition of H-Ras or N-Ras (see picture). Also endogenous Ras only exhibits one band and not two which is a hint for the recognition of endogenous N-Ras next to K-Ras.In immunofluorescence experiments antibody works awesome at a dilution of 1:100 for pancreatic cancer cell lines. In total I'm very satisfied, but you have to use more antibody than written in the data sheet.
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