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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KREMEN1 Polyclonal antibody (25434-1-AP)

    WB analysis using 25434-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25434-1-AP (KREMEN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

KREMEN1 Polyclonal antibody

KREMEN1 Polyclonal Antibody for WB, ELISA
Cat No. 25434-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Dickkopf receptor, KREMEM1, KREMEN, Kremen protein 1, KREMEN1, KRM1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25434-1-AP targets KREMEN1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KREMEN1 fusion protein Ag22092 相同性解析による交差性が予測される生物種
Full Name kringle containing transmembrane protein 1
Calculated molecular weight 473 aa, 52 kDa
Observed molecular weight50 kDa
GenBank accession numberBC063787
Gene Symbol KREMEN1
Gene ID (NCBI) 83999
RRIDAB_3669475
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MU8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kringle containing transmembrane protein 1 (KREMEN1, also known as KRM1, ECTD13, and KREMEN) is a transmembrane receptor located in the membrane. During the development of various organs, including the nervous system, limbs, and liver, KREMEN1 synergizes with the Dkk proteins to inhibit Wnt signaling (PMID: 18505822). KREMEN1 has been reported as a tumor suppressor, preventing cancer cell survival in a ligand-poor environment (PMID: 31069116). Variations and mutations of KREMEN1 have been associated with ectodermal dysplasia and Hand-foot-and-mouth disease (PMID: 34028942; 31911601).

Protocols

Product Specific Protocols
WB protocol for KREMEN1 antibody 25434-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols