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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using KRR1 Polyclonal antibody (12772-1-AP)

    WB analysis of human brain using 12772-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12772-1-AP (KRR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using KRR1 Polyclonal antibody (12772-1-AP)

    WB analysis of human kidney using 12772-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12772-1-AP (KRR1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KRR1 Polyclonal antibody (12772-1-AP)

    IF Staining of HepG2 using 12772-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12772-1-AP (KRR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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KRR1 Polyclonal antibody

KRR1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 12772-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Rip-1, RIP 1, Rev-interacting protein 1, KRR-R motif-containing protein 1, HRB2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman brain tissue, human kidney tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12772-1-AP targets KRR1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KRR1 fusion protein Ag3464 相同性解析による交差性が予測される生物種
Full Name KRR1, small subunit (SSU) processome component, homolog (yeast)
Calculated molecular weight 381 aa, 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC026107
Gene Symbol KRR1
Gene ID (NCBI) 11103
RRIDAB_2134283
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13601
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KRR1 antibody 12772-1-APDownload protocol
IF protocol for KRR1 antibody 12772-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols