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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Cytokeratin 1-specific Recombinant antibody

Cytokeratin 1-specific Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230261F9

Cat no : CL488-82972

Synonyms

KRT1-specific, Cytokeratin 1, Cytokeratin-1, Cytokeratin1, Cytokeratin,Keratin

Validation Data Gallery

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  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated KRT1-specific Recomb (CL488-82972)

    FC experiment of MCF-7 using CL488-82972

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Krt1-Specific Recombinant Antibody (CL488-82972, Clone:230261F9)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated KRT1-specific Recomb (CL488-82972)

    FC experiment of A431 using CL488-82972

    1x10^6 A431 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Krt1-Specific Recombinant Antibody (CL488-82972, Clone:230261F9)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inA431 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82972 targets Cytokeratin 1-specific in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name keratin 1
Calculated molecular weight 66 kDa
Observed molecular weight 67 kDa
GenBank accession numberNM_006121
Gene symbol KRT1
Gene ID (NCBI) 3848
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin-1 is type II keratin. It is a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Cytokeratin 1-specific antibody CL488-82972Download protocol
Standard Protocols
Click here to view our Standard Protocols