KRT12 Polyclonal antibody
KRT12 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 24789-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse eye tissue |
Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IF | See 6 publications below |
Product Information
24789-1-AP targets KRT12 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | KRT12 fusion protein Ag20312 相同性解析による交差性が予測される生物種 |
Full Name | keratin 12 |
Calculated molecular weight | 494 aa, 54 kDa |
Observed molecular weight | 50-55 kDa |
GenBank accession number | BC156641 |
Gene symbol | KRT12 |
Gene ID (NCBI) | 3859 |
RRID | AB_2879726 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for KRT12 antibody 24789-1-AP | Download protocol |
IHC protocol for KRT12 antibody 24789-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Invest Ophthalmol Vis Sci Wounding Induces Facultative Opn5-Dependent Circadian Photoreception in the Murine Cornea. | ||
Exp Eye Res Low-dose repeated exposure to chemical surfactant impairs corneal epithelium: When personal cleaning products entering the eye. | ||
Int Ophthalmol ZD6474 attenuates TGF-β1-induced fibrosis in human Tenon fibroblasts and inhibits neovascularization via AKT-mTOR signaling pathway | ||
J Ocul Pharmacol Ther ZD6474 Attenuates Fibrosis and Inhibits Neovascularization in Human Pterygium by Suppressing AKT-mTOR Signaling Pathway | ||
Transl Vis Sci Technol Unraveling the Intricate Network of lncRNAs in Corneal Epithelial Wound Healing: Insights Into the Regulatory Role of linc17500 |