KRT12 Polyclonal antibody

KRT12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24789-1-AP

Synonyms

CK 12, Cytokeratin 12, K12, keratin 12, KRT12


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Tested Applications

Positive WB detected inmouse eye tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 6 publications below

Product Information

24789-1-AP targets KRT12 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KRT12 fusion protein Ag20312 相同性解析による交差性が予測される生物種
Full Name keratin 12
Calculated molecular weight 494 aa, 54 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC156641
Gene symbol KRT12
Gene ID (NCBI) 3859
RRIDAB_2879726
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KRT12 antibody 24789-1-APDownload protocol
IHC protocol for KRT12 antibody 24789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

EBioMedicine

Cisd2 plays an essential role in corneal epithelial regeneration.

Authors - Chi-Chin Sun
mouseIF

Invest Ophthalmol Vis Sci

Wounding Induces Facultative Opn5-Dependent Circadian Photoreception in the Murine Cornea.

Authors - Nicolás M Díaz
mouseIF

Exp Eye Res

Low-dose repeated exposure to chemical surfactant impairs corneal epithelium: When personal cleaning products entering the eye.

Authors - Jie Wu
humanIF

Int Ophthalmol

ZD6474 attenuates TGF-β1-induced fibrosis in human Tenon fibroblasts and inhibits neovascularization via AKT-mTOR signaling pathway

Authors - Wenting Liu
humanIF

J Ocul Pharmacol Ther

ZD6474 Attenuates Fibrosis and Inhibits Neovascularization in Human Pterygium by Suppressing AKT-mTOR Signaling Pathway

Authors - Wenting Liu
mouseIF

Transl Vis Sci Technol

Unraveling the Intricate Network of lncRNAs in Corneal Epithelial Wound Healing: Insights Into the Regulatory Role of linc17500

Authors - Qiongjie Cao