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  • Featured Product
  • KD/KO Validated

Cytokeratin 13 Recombinant antibody, PBS Only (Capture)

Cytokeratin 13 Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230366B10

Cat no : 83058-1-PBS

Synonyms

KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 13 Recombinant antibody (83058-1-RR)

    WB analysis using 83058-1-RR (same clone as 83058-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83058-1-RR (KRT13 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using Cytokeratin 13 Recombinant antibody (83058-1-RR)

    WB analysis of A431 using 83058-1-RR (same clone as 83058-1-PBS)

    WB result of Cytokeratin 13 antibody (83058-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 13 transfected A431 cells. This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00074-2

    Sandwich ELISA standard curve of MP00074-2

    Sandwich ELISA standard curve of MP00074-2, Human Cytokeratin 13 Recombinant Matched Antibody Pair - PBS only. 83058-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0217. 83058-5-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP00074-2

    Sample test of MP00074-2

    The mean Cytokeratin 13 concentration was determined to be 28.00 ng/mL in A431 cell extract based on a 1.50 mg/mL extract load and 12.84 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Cytokeratin 13 Recombinant antibody (83058-1-RR)

    IHC staining of human urothelial carcinoma using 83058-1-RR (same clone as 83058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Cytokeratin 13 Recombinant antibody (83058-1-RR)

    IHC staining of human urothelial carcinoma using 83058-1-RR (same clone as 83058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 13 Recombinant antibody (83058-1-RR)

    IHC staining of human oesophagus cancer using 83058-1-RR (same clone as 83058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 13 Recombinant antibody (83058-1-RR)

    IHC staining of human oesophagus cancer using 83058-1-RR (same clone as 83058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical squamous cancer tissue using Cytokeratin 13 Recombinant antibody (83058-1-RR)

    IHC staining of human cervical squamous cancer using 83058-1-RR (same clone as 83058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical squamous cancer tissue using Cytokeratin 13 Recombinant antibody (83058-1-RR)

    IHC staining of human cervical squamous cancer using 83058-1-RR (same clone as 83058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skin tissue using Cytokeratin 13 Recombinant antibody (83058-1-RR)

    IHC staining of rat skin using 83058-1-RR (same clone as 83058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skin tissue using Cytokeratin 13 Recombinant antibody (83058-1-RR)

    IHC staining of rat skin using 83058-1-RR (same clone as 83058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Cytokeratin 13 Recombinant antibody (83058-1-RR)

    IHC staining of mouse skin using 83058-1-RR (same clone as 83058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Cytokeratin 13 Recombinant antibody (83058-1-RR)

    IHC staining of mouse skin using 83058-1-RR (same clone as 83058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using Cytokeratin 13 Recombinant antibody (83058-1-RR)

    IF Staining of HaCaT using 83058-1-RR (same clone as 83058-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 13 antibody (83058-1-RR, Clone: 230366B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00074-1

    Cytometric bead array standard curve of MP00074-1

    Cytometric bead array standard curve of MP00074-1, Cytokeratin 13 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83058-1-PBS. Detection antibody: 83058-3-PBS. Standard: Ag0217. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83058-1-RR

    Affinity and Kinetic Characterization of 83058-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83058-1-RR against Human Cytokeratin 13 were performed. The affinity constant is 1.99 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83058-1-PBS targets Cytokeratin 13 as part of a matched antibody pair:

MP00074-1: 83058-1-PBS capture and 83058-3-PBS detection (validated in Cytometric bead array)

MP00074-2: 83058-1-PBS capture and 83058-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cytokeratin 13 fusion protein Ag0217 相同性解析による交差性が予測される生物種
Full Name keratin 13
Calculated molecular weight 50 kDa
Observed molecular weight50 kDa
GenBank accession numberBC002661
Gene symbol Cytokeratin 13
Gene ID (NCBI) 3860
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.