Cytokeratin 15 Polyclonal antibody

Name: Cytokeratin 15 Polyclonal antibody
Brand: Proteintech
SKU: 10137-1-AP

Cytokeratin 15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10137-1-AP

Synonyms

KRT15, keratin 15, Cytokeratin-15 , Cytokeratin,Keratin, CK-15

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of A431 using 10137-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 10137-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10137-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10137-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 10137-1-AP
IP result of anti-Cytokeratin 15 (IP:10137-1-AP, 4ug; Detection:10137-1-AP 1:8000) with A431 cells lysate 1000 ug.
IHC staining of human cervical cancer using 10137-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 10137-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 10137-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10137-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10137-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 10137-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10137-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 15 antibody (10137-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Cat No. 10137-1-AP

Size/Concentration

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, mouse thymus tissue, HeLa cells, PC-3 cells
Positive IP detected in	A431 cells
Positive IHC detected in	human cervical cancer tissue, human oesophagus cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 6 publications below
IF	See 9 publications below

Product Information

10137-1-AP targets Cytokeratin 15 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cytokeratin 15 fusion protein Ag0185 相同性解析による交差性が予測される生物種
Full Name	keratin 15
Calculated molecular weight	49 kDa
Observed molecular weight	49 kDa
GenBank accession number	BC002641
Gene symbol	KRT15
Gene ID (NCBI)	3866
RRID	AB_2234359
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 15 antibody 10137-1-AP	Download protocol
IHC protocol for Cytokeratin 15 antibody 10137-1-AP	Download protocol
IF protocol for Cytokeratin 15 antibody 10137-1-AP	Download protocol
IP protocol for Cytokeratin 15 antibody 10137-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Biomater Sci A hybrid hydrogel composed of chitin and β-glucan for the effective management of wound healing and scarring Authors - Ximeng Kang View Article
mouse	IF	Stem Cells LIM Homeobox Domain 2 (Lhx2) is required for corneal epithelial homeostasis. Authors - R Sartaj View Article
human	IF	J Invest Dermatol GW bodies: cytoplasmic compartments in normal human skin. Authors - Zee Jennifer M JM View Article
human	WB	Carcinogenesis Survival Of Skin Cancer Stem Cells Requires The Ezh2 Polycomb Group Protein. Authors - Gautam Adhikary View Article
mouse	IF	Sci Rep Characterization of slow cycling corneal limbal epithelial cells identifies putative stem cell markers. Authors - R Sartaj View Article
rat	WB,IF	Front Med (Lausanne) Metformin Promotes Mechanical Stretch-Induced Skin Regeneration by Improving the Proliferative Activity of Skin-Derived Stem Cells. Authors - Shaoheng Xiong View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 15 Polyclonal antibody

Cytokeratin 15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of A431 using 10137-1-AP

WB analysis of mouse thymus using 10137-1-AP

WB analysis of HeLa using 10137-1-AP

WB analysis of PC-3 using 10137-1-AP

IP experiment of A431 using 10137-1-AP

IHC staining of human cervical cancer using 10137-1-AP

IHC staining of human cervical cancer using 10137-1-AP

IHC staining of human oesophagus cancer using 10137-1-AP

IHC staining of human breast cancer using 10137-1-AP

IHC staining of human breast cancer using 10137-1-AP

IHC staining of human oesophagus cancer using 10137-1-AP

IF Staining of HeLa using 10137-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A hybrid hydrogel composed of chitin and β-glucan for the effective management of wound healing and scarring

LIM Homeobox Domain 2 (Lhx2) is required for corneal epithelial homeostasis.

GW bodies: cytoplasmic compartments in normal human skin.

Survival Of Skin Cancer Stem Cells Requires The Ezh2 Polycomb Group Protein.

Characterization of slow cycling corneal limbal epithelial cells identifies putative stem cell markers.

Metformin Promotes Mechanical Stretch-Induced Skin Regeneration by Improving the Proliferative Activity of Skin-Derived Stem Cells.