Cytokeratin 18 Recombinant antibody
Cytokeratin 18 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
4F2
Cat no : 82817-5-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, A549 cells, HCT 116 cells, HepG2 cells, K-562 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human appendicitis tissue, human breast cancer tissue, human liver tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82817-5-RR targets Cytokeratin 18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Cytokeratin 18 fusion protein Ag1260 相同性解析による交差性が予測される生物種 |
| Full Name | keratin 18 |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC000180 |
| Gene symbol | Cytokeratin 18 |
| Gene ID (NCBI) | 3875 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cytokeratin 18, also known as CK18, is a type I intermediate filament protein which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. CK18 and its coexpressed complementary type II keratin partner, CK8, are persistently expressed in a variety of adult epithelial organs, such as the liver, lung, kidney, pancreas, gastrointestinal tract, and mammary gland, and are also expressed by cancers that arise from these tissues. In the absence of CK8, the CK18 protein is degraded and keratin intermediate filaments are not formed.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 18 antibody 82817-5-RR | Download protocol |
| IHC protocol for Cytokeratin 18 antibody 82817-5-RR | Download protocol |
| IF protocol for Cytokeratin 18 antibody 82817-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
