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  • Featured Product
  • KD/KO Validated

Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14965-1-AP

Synonyms

CK19, CK 19, KRT19, K19, Cytokeratin-19

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    WB analysis of MCF-7 using 14965-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    WB analysis of HeLa using 14965-1-AP

    WB result of KRT19 antibody (14965-1-AP, 1:50,000) with si-Control and si-KRT19 transfected HeLa cells.

  • Western Blot (WB) analysis of HepG2 cells using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    WB analysis of HepG2 using 14965-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    WB analysis of BxPC-3 using 14965-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    IHC staining of human colon using 14965-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brown disease using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    IHC staining of human brown disease using 14965-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brown disease using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    IHC staining of human brown disease using 14965-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    IHC staining of human colon using 14965-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    IHC staining of human liver cancer using 14965-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    IHC staining of human liver cancer using 14965-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    IHC staining of human lung cancer using 14965-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    IHC staining of human lung cancer using 14965-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    IHC staining of human liver cancer using 14965-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    IHC staining of human liver cancer using 14965-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    IHC staining of rat colon using 14965-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    IHC staining of rat colon using 14965-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    IHC staining of mouse colon using 14965-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    IHC staining of mouse colon using 14965-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    IHC staining of human cervical cancer using 14965-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    IHC staining of human cervical cancer using 14965-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    IF Staining of HeLa using 14965-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cytokeratin 19 antibody (14965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    IF Staining of A431 using 14965-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 19 antibody (14965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

  • Flow cytometry (FC) experiment of MCF-7 cells using Cytokeratin 19 Polyclonal antibody (14965-1-AP)

    FC experiment of MCF-7 using 14965-1-AP

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug Cytokeratin 19 Polyclonal antibody (14965-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inMCF7 cells, HeLa cells, HepG2 cells, BxPC-3 cells
Positive IHC detected inhuman colon tissue, human liver cancer tissue, human cervical cancer tissue, human brown disease, mouse colon tissue, rat colon tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, A431 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14965-1-AP targets Cytokeratin 19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 19 fusion protein Ag6830 相同性解析による交差性が予測される生物種
Full Name keratin 19
Calculated molecular weight 44 kDa
Observed molecular weight 40 kDa, 46 kDa
GenBank accession numberBC002539
Gene symbol Cytokeratin 19
Gene ID (NCBI) 3880
RRIDAB_2133324
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. This antibody, generated against full length KRT19 protein, also recognizes KRT17, another type I keratin homologous to KRT19.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 19 antibody 14965-1-APDownload protocol
IHC protocol for Cytokeratin 19 antibody 14965-1-APDownload protocol
IF protocol for Cytokeratin 19 antibody 14965-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Gastroenterology

ECM1 Prevents Activation of Transforming Growth Factor β, Hepatic Stellate Cells, and Fibrogenesis in Mice.

Authors - Weiguo Fan
View Article
mouseIF

Hepatology

Cholestasis-induced adaptive remodeling of interlobular bile ducts.

Authors - Nachiket Vartak
View Article
monkeyIHC,IF

Int J Biol Sci

Hepatobiliary organoids differentiated from hiPSCs relieve cholestasis-induced liver fibrosis in nonhuman primates

Authors - Hongmei Li
View Article
humanIHC

Cell Rep

In Vivo Functional Platform Targeting Patient-Derived Xenografts Identifies WDR5-Myc Association as a Critical Determinant of Pancreatic Cancer.

Authors - Alessandro Carugo
View Article
mouseIHC

Arch Toxicol

Galunisertib modifies the liver fibrotic composition in the Abcb4Ko mouse model.

Authors - Seddik Hammad
View Article
mouseIF

Front Cell Dev Biol

Volumetric imaging reveals VEGF-C-dependent formation of hepatic lymph vessels in mice

Authors - Stefanie Bobe
View Article