Cytokeratin 2e Polyclonal antibody
Cytokeratin 2e Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 21725-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, mouse skin tissue, rat skin tissue. |
Positive IHC detected in | human cervical cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IF | See 1 publications below |
Product Information
21725-1-AP targets Cytokeratin 2e in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Cytokeratin 2e fusion protein Ag16384 相同性解析による交差性が予測される生物種 |
Full Name | keratin 2 |
Calculated molecular weight | 639 aa, 66 kDa |
Observed molecular weight | 65 kDa |
GenBank accession number | BC096294 |
Gene symbol | KRT2 |
Gene ID (NCBI) | 3849 |
RRID | AB_2878914 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 2 is a type II cytokeratin. It is found largely in the upper spinous layer of epidermal keratinocytes, and also in other regions, notably the epidermis covering the knee, thigh, and groin.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Cytokeratin 2e antibody 21725-1-AP | Download protocol |
IHC protocol for Cytokeratin 2e antibody 21725-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cont Lens Anterior Eye The biomechanical proteins different between low myopic corneas and moderate to high myopic corneas in human |