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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 20 Recombinant antibody

Cytokeratin 20 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2H10

Cat no : 82428-1-RR

Synonyms

KRT20, keratin 20, K20, Cytokeratin-20, CK-20

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 20 Recombinant antibody (82428-1-RR)

    WB analysis using 82428-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:22000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using Cytokeratin 20 Recombinant antibody (82428-1-RR)

    WB analysis of COLO 320 using 82428-1-RR

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:22000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cytokeratin 20 Recombinant antibody (82428-1-RR)

    IHC staining of human colon cancer using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cytokeratin 20 Recombinant antibody (82428-1-RR)

    IHC staining of human colon cancer using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 20 Recombinant antibody (82428-1-RR)

    IHC staining of human colon using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 20 Recombinant antibody (82428-1-RR)

    IHC staining of human colon using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using Cytokeratin 20 Recombinant antibody (82428-1-RR)

    IHC staining of human appendicitis using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using Cytokeratin 20 Recombinant antibody (82428-1-RR)

    IHC staining of human appendicitis using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Cytokeratin 20 Recombinant antibody (82428-1-RR)

    IHC staining of human urothelial carcinoma using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Cytokeratin 20 Recombinant antibody (82428-1-RR)

    IHC staining of human urothelial carcinoma using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using Cytokeratin 20 Recombinant antibody (82428-1-RR)

    IF Staining of HT-29 using 82428-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HT-29 cells using Cytokeratin 20 Recombinant antibody (82428-1-RR)

    FC experiment of HT-29 using 82428-1-RR

    1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 20 (82428-1-RR, Clone:2H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82428-1-RR

    Affinity and Kinetic Characterization of 82428-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82428-1-RR against Human Cytokeratin 20 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHT-29 cells, COLO 320 cells, pig colon tissue, mouse colon tissue, rat colon tissue
Positive IHC detected inhuman colon cancer tissue, human appendicitis tissue, human colon tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHT-29 cells
Positive FC (Intra) detected inHT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82428-1-RR targets Cytokeratin 20 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cytokeratin 20 fusion protein Ag11308 相同性解析による交差性が予測される生物種
Full Name keratin 20
Calculated molecular weight 424 aa, 48 kDa
Observed molecular weight46 kDa
GenBank accession numberBC031559
Gene symbol Cytokeratin 20
Gene ID (NCBI) 54474
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 20 antibody 82428-1-RRDownload protocol
IHC protocol for Cytokeratin 20 antibody 82428-1-RRDownload protocol
IF protocol for Cytokeratin 20 antibody 82428-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols