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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KRT23 Polyclonal antibody

KRT23 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24049-1-AP

Synonyms

K23, Cytokeratin-23, Cytokeratin 23 , CK-23, CK23

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using KRT23 Polyclonal antibody (24049-1-AP)

    WB analysis of BxPC-3 using 24049-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 24049-1-AP (KRT23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using KRT23 Polyclonal antibody (24049-1-AP)

    WB analysis of HeLa using 24049-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24049-1-AP (KRT23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using KRT23 Polyclonal antibody (24049-1-AP)

    WB analysis of HepG2 using 24049-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24049-1-AP (KRT23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KRT23 Polyclonal antibody (24049-1-AP)

    IHC staining of human colon cancer using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KRT23 Polyclonal antibody (24049-1-AP)

    IHC staining of human colon cancer using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using KRT23 Polyclonal antibody (24049-1-AP)

    IHC staining of human liver cancer using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using KRT23 Polyclonal antibody (24049-1-AP)

    IHC staining of human liver cancer using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using KRT23 Polyclonal antibody (24049-1-AP)

    IHC staining of rat colon using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using KRT23 Polyclonal antibody (24049-1-AP)

    IHC staining of rat colon using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using KRT23 Polyclonal antibody (24049-1-AP)

    IHC staining of human bowen disease using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using KRT23 Polyclonal antibody (24049-1-AP)

    IHC staining of human bowen disease using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using KRT23 Polyclonal antibody (24049-1-AP)

    IHC staining of mouse colon using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using KRT23 Polyclonal antibody (24049-1-AP)

    IHC staining of mouse colon using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using KRT23 Polyclonal antibody (24049-1-AP)

    IHC staining of human placenta using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using KRT23 Polyclonal antibody (24049-1-AP)

    IHC staining of human placenta using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using KRT23 Polyclonal antibody (24049-1-AP)

    IHC staining of human skin cancer using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using KRT23 Polyclonal antibody (24049-1-AP)

    IHC staining of human skin cancer using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using KRT23 Polyclonal antibody (24049-1-AP)

    IHC staining of human skin cancer using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using KRT23 Polyclonal antibody (24049-1-AP)

    IHC staining of human skin cancer using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inBxPC-3 cells, HepG2 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue, human skin cancer tissue, human placenta tissue, human liver cancer tissue, rat colon tissue, mouse colon tissue, human bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

24049-1-AP targets KRT23 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KRT23 fusion protein Ag21129 相同性解析による交差性が予測される生物種
Full Name keratin 23 (histone deacetylase inducible)
Calculated molecular weight 422 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC028356
Gene symbol KRT23
Gene ID (NCBI) 25984
RRIDAB_2879416
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT23 belongs to type I keratins.

Protocols

Product Specific Protocols
WB protocol for KRT23 antibody 24049-1-APDownload protocol
IHC protocol for KRT23 antibody 24049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Rep

Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model.

Authors - Masatoshi Ohgushi
View Article