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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 3 Polyclonal antibody

Cytokeratin 3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16850-1-AP

Synonyms

Cytokeratin 3-specific, KRT3, keratin 3, Cytokeratin-3, CK-3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 3 Polyclonal antibody (16850-1-AP)

    WB analysis using 16850-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16850-1-AP (Cytokeratin 3-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA431 cells, HeLa cells, Y79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16850-1-AP targets Cytokeratin 3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name keratin 3
Calculated molecular weight 64 kDa
Observed molecular weight68 kDa
GenBank accession numberNM_057088
Gene symbol Cytokeratin 3
Gene ID (NCBI) 3850
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a member of the type II cytokeratin subfamily, KRT3 is expressed together with KRT12 during 'corneal-type' differentiation.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 3 antibody 16850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols