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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using KRT33B Polyclonal antibody (11048-1-AP)

    WB analysis of MCF-7 using 11048-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11048-1-AP (KRT33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using KRT33B Polyclonal antibody (11048-1-AP)

    WB analysis of human brain using 11048-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11048-1-AP (KRT33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using KRT33B Polyclonal antibody (11048-1-AP)

    IHC staining of human skin cancer using 11048-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using KRT33B Polyclonal antibody (11048-1-AP)

    IHC staining of human skin cancer using 11048-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KRT33B Polyclonal antibody (11048-1-AP)

    IHC staining of human prostate cancer using 11048-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KRT33B Polyclonal antibody (11048-1-AP)

    IHC staining of human prostate cancer using 11048-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KRT33B Polyclonal antibody (11048-1-AP)

    IHC staining of human prostate cancer using 11048-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KRT33B Polyclonal antibody (11048-1-AP)

    IHC staining of human prostate cancer using 11048-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using KRT33B Polyclonal antibody (11048-1-AP)

    IF Staining of MCF-7 using 11048-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 11048-1-AP (KRT33B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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KRT33B Polyclonal antibody

KRT33B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11048-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Keratin-33B, Keratin, type I cuticular Ha3-II, keratin 33B, K33B, HHA3-II

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, human brain tissue
Positive IHC detected inhuman skin cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11048-1-AP targets KRT33B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KRT33B fusion protein Ag1522 相同性解析による交差性が予測される生物種
Full Name keratin 33B
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC009971
Gene Symbol KRT33B
Gene ID (NCBI) 3884
RRIDAB_2133874
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14525
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 33 belongs to type I keratin and is found in hair and nails. There are two isoforms of this protein, encoded by two separate genes, KRTHA3A and KRTHA3B.

Protocols

Product Specific Protocols
WB protocol for KRT33B antibody 11048-1-APDownload protocol
IHC protocol for KRT33B antibody 11048-1-APDownload protocol
IF protocol for KRT33B antibody 11048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Asian J Androl

PMID: 24994782

Authors - Yan-Min Ma
View Article