Cytokeratin 4 Polyclonal antibody

Cytokeratin 4 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 16572-1-AP

Synonyms

KRT4, Cytokeratin-4, Cytokeratin,Keratin, CYK4, CK-4


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Tested Applications

Positive WB detected inA431 cells, human bladder tissue
Positive IHC detected inhuman cervical cancer tissue, human bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skin tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16572-1-AP targets Cytokeratin 4 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 4 fusion protein Ag9854 相同性解析による交差性が予測される生物種
Full Name keratin 4
Calculated molecular weight 534 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC042174
Gene symbol Cytokeratin 4
Gene ID (NCBI) 3851
RRIDAB_2134041
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 4 is a type II cytokeratins. It is specifically found in differentiated layers of the mucosal and esophageal epithelia together with keratin 13.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 4 antibody 16572-1-APDownload protocol
IHC protocol for Cytokeratin 4 antibody 16572-1-APDownload protocol
IF protocol for Cytokeratin 4 antibody 16572-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nature

A single-cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyte.

Authors - Lindsey W Plasschaert
IF

Development

Novel dynamics of human mucociliary differentiation revealed by single-cell RNA sequencing of nasal epithelial cultures.

Authors - Sandra Ruiz García
humanIHC

Int J Mol Sci

Early Clinical Outcomes of the First Commercialized Human Autologous Ex Vivo Cultivated Oral Mucosal Epithelial Cell Transplantation for Limbal Stem Cell Deficiency: Two Case Reports and Literature Review

Authors - Hiroshi Toshida
humanIHC

Ann Diagn Pathol

The molecular-based differentiation of Heck's disease from its mimics including oral condyloma and white sponge nevus.

Authors - Ziv Schwartz
mouseIF

Cell Rep

An Immunocompetent Mouse Model of HPV16(+) Head and Neck Squamous Cell Carcinoma.

Authors - Miranda B Carper
humanWB

Oncotarget

Significance of the NOR1-FOXA1/HDAC2-Slug regulatory network in epithelial-mesenchymal transition of tumor cells.

Authors - Wei Wang