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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 6A-specific Polyclonal antibody

Cytokeratin 6A-specific Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16853-1-AP

Synonyms

Cytokeratin 6, KRT6A, CK-6D, CK-6A, CK6A

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    WB analysis of A431 using 16853-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    WB analysis of mouse skin using 16853-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    WB analysis of HeLa using 16853-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human saliva using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    WB analysis of human saliva using 16853-1-AP

    human saliva were subjected to SDS PAGE followed by western blot with 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    IP experiment of HeLa using 16853-1-AP

    IP result of anti-Cytokeratin 6A-specific (IP:16853-1-AP, 4ug; Detection:16853-1-AP 1:300) with HeLa cells lysate 520ug.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    IHC staining of human skin cancer using 16853-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    IHC staining of human skin cancer using 16853-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    IHC staining of human bowen disease using 16853-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    IHC staining of human bowen disease using 16853-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA431 cells, mouse skin tissue, HeLa cells, human saliva tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman skin cancer tissue, human bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16853-1-AP targets Cytokeratin 6A-specific in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name keratin 6A
Calculated molecular weight 60 kDa
Observed molecular weight 56 kDa
GenBank accession numberNM_005554
Gene symbol Cytokeratin 6A
Gene ID (NCBI) 3853
RRIDAB_2134304
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence. This antibody is against all three isoforms of keratin 6, but it does not bind other keratins.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 6A-specific antibody 16853-1-APDownload protocol
IHC protocol for Cytokeratin 6A-specific antibody 16853-1-APDownload protocol
IP protocol for Cytokeratin 6A-specific antibody 16853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols