Cytokeratin 6A Polyclonal antibody

Name: Cytokeratin 6A Polyclonal antibody
Brand: Proteintech
SKU: 10590-1-AP

Cytokeratin 6A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10590-1-AP

Synonyms

Cytokeratin 6, KRT6A, CK-6D, CK-6A, CK6A

Validation Data Gallery

Filter:

AllWBIHCIF/ICCFC (Intra)

WB analysis of A431 using 10590-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10590-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 10590-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat skin using 10590-1-AP
rat skin tissue were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of U-251 using 10590-1-AP
U-251 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10590-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10590-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human oesophagus cancer using 10590-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 10590-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10590-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10590-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin using 10590-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 10590-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 10590-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody at dilution of 1:200 (under 10x lens).
IHC staining of human lung cancer using 10590-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody at dilution of 1:200 (under 40x lens).
IF Staining of A431 using 10590-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 6A antibody (10590-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of A431 using 10590-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 10590-1-AP (Cytokeratin 6A antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 10590-1-AP
Immunofluorescent analysis of Hela cells, using KRT6A antibody 10590-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
FC experiment of A431 using 10590-1-AP
1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 6A (10590-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 10590-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, MCF-7 cells, HeLa cells, A375 cells, rat skin tissue, U-251 cells
Positive IHC detected in	human oesophagus cancer tissue, human breast cancer tissue, human skin tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells, HeLa cells
Positive FC (Intra) detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:10000-1:50000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 11 publications below
IHC	See 7 publications below
IF	See 8 publications below

Product Information

10590-1-AP targets Cytokeratin 6A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cytokeratin 6A fusion protein Ag0882 相同性解析による交差性が予測される生物種
Full Name	keratin 6A
Calculated molecular weight	60 kDa
Observed molecular weight	56 kDa
GenBank accession number	BC008807
Gene symbol	Cytokeratin 6A
Gene ID (NCBI)	3853
RRID	AB_2134306
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three are three keratin 6 isoforms: ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 6A antibody 10590-1-AP	Download protocol
IHC protocol for Cytokeratin 6A antibody 10590-1-AP	Download protocol
IF protocol for Cytokeratin 6A antibody 10590-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Immunity Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis. Authors - Fangzhou Lou View Article
		Cell Rep Keratinocyte FABP5-VCP complex mediates recruitment of neutrophils in psoriasis Authors - Jiaqing Hao View Article
human,mouse	IF	Cell Rep Collagen 1-mediated CXCL1 secretion in tumor cells activates fibroblasts to promote radioresistance of esophageal cancer Authors - Xinyu Yang View Article
mouse	WB	Phytother Res Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization Authors - Yongling Ning View Article
human,mouse	WB,IHC,IF	J Cell Mol Med Ozone therapy promotes the differentiation of basal keratinocytes via increasing Tp63-mediated transcription of KRT10 to improve psoriasis. Authors - Lihua Gao View Article
human	WB	J Eur Acad Dermatol Venereol Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi. Authors - T Yuan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 6A Polyclonal antibody

Cytokeratin 6A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis of A431 using 10590-1-AP

WB analysis of MCF-7 using 10590-1-AP

WB analysis of A375 using 10590-1-AP

WB analysis of rat skin using 10590-1-AP

WB analysis of U-251 using 10590-1-AP

WB analysis of MCF-7 using 10590-1-AP

WB analysis of HeLa using 10590-1-AP

IHC staining of human oesophagus cancer using 10590-1-AP

IHC staining of human oesophagus cancer using 10590-1-AP

IHC staining of human breast cancer using 10590-1-AP

IHC staining of human breast cancer using 10590-1-AP

IHC staining of human skin using 10590-1-AP

IHC staining of human skin using 10590-1-AP

IHC staining of human lung cancer using 10590-1-AP

IHC staining of human lung cancer using 10590-1-AP

IF Staining of A431 using 10590-1-AP

IF Staining of A431 using 10590-1-AP

IF Staining of HeLa using 10590-1-AP

FC experiment of A431 using 10590-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.

Keratinocyte FABP5-VCP complex mediates recruitment of neutrophils in psoriasis

Collagen 1-mediated CXCL1 secretion in tumor cells activates fibroblasts to promote radioresistance of esophageal cancer

Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization

Ozone therapy promotes the differentiation of basal keratinocytes via increasing Tp63-mediated transcription of KRT10 to improve psoriasis.

Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.