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  • Featured Product
  • KD/KO Validated

Cytokeratin 7-specific Polyclonal antibody

Cytokeratin 7-specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 17513-1-AP

Synonyms

Cytokeratin 7, KRT7, Cytokeratin-7, Cytokeratin,Keratin, CK-7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    WB analysis using 17513-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of T-47D cells using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    WB analysis of T-47D using 17513-1-AP

    T-47D cells were subjected to SDS PAGE followed by western blot with 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    WB analysis of HeLa using 17513-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17513-1-AP (Cytokeratin 7-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IP experiment of HepG2 using 17513-1-AP

    IP result of anti-Cytokeratin 7-specific (IP:17513-1-AP, 4ug; Detection:17513-1-AP 1:1000) with HepG2 cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IHC staining of human lung cancer using 17513-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IHC staining of human lung cancer using 17513-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IHC staining of human lung cancer using 17513-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IHC staining of human bowen disease using 17513-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IHC staining of human bowen disease using 17513-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IHC staining of human ovary tumor using 17513-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IHC staining of human ovary tumor using 17513-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IHC staining of human kidney using 17513-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IHC staining of human kidney using 17513-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IHC staining of human breast cancer using 17513-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). heat mediated antigen retrieved with .

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IHC staining of human breast cancer using 17513-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). heat mediated antigen retrieved with .

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IHC staining of human cervical cancer using 17513-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 17513-1-AP (KRT7-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IHC staining of human cervical cancer using 17513-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 17513-1-AP (KRT7-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IHC staining of human kidney using 17513-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IHC staining of human kidney using 17513-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IF Staining of HepG2 using 17513-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IF Staining of HepG2 using 17513-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IF Staining of HeLa using 17513-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    IF Staining of HepG2 using 17513-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

    FC experiment of HeLa using 17513-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 7-specific (17513-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA431 cells, HeLa cells, T-47D cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue, human bowen disease, human breast cancer tissue, human cervical cancer tissue, human kidney tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17513-1-AP targets Cytokeratin 7-specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name keratin 7
Calculated molecular weight 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberNM_005556
Gene symbol Cytokeratin 7
Gene ID (NCBI) 3855
RRIDAB_2134468
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. This antibody is specifically against KRT7.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 7-specific antibody 17513-1-APDownload protocol
IHC protocol for Cytokeratin 7-specific antibody 17513-1-APDownload protocol
IF protocol for Cytokeratin 7-specific antibody 17513-1-APDownload protocol
IP protocol for Cytokeratin 7-specific antibody 17513-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Transl Res

SERPINA5 may promote the development of preeclampsia by disruption of the uPA/uPAR pathway.

Authors - Yan Long
View Article
humanIHC

Clin Cancer Res

Integrative Genomic Analysis of Gemcitabine Resistance in Pancreatic Cancer by Patient-derived Xenograft Models.

Authors - Gang Yang
View Article
mouseIHC

Angiogenesis

A novel lymphatic pattern promotes metastasis of cervical cancer in a hypoxic tumour-associated macrophage-dependent manner.

Authors - Xiao-Jing Chen
View Article
ratIHC,IF

Proc Natl Acad Sci U S A

Up-regulation of CD81 inhibits cytotrophoblast invasion and mediates maternal endothelial cell dysfunction in preeclampsia.

Authors - Li Shen
View Article
mouseIF

Cell Prolif

Insufficient GDF15 expression predisposes women to unexplained recurrent pregnancy loss by impairing extravillous trophoblast invasion

Authors - Chunzi Lyu
View Article
mouseIHC

Oncogene

USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model.

Authors - Juntae Kwon
View Article