Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis using 17513-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
WB analysis using 17513-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of T-47D using 17513-1-AP
T-47D cells were subjected to SDS PAGE followed by western blot with 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17513-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17513-1-AP (Cytokeratin 7-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 17513-1-AP
IP result of anti-Cytokeratin 7-specific (IP:17513-1-AP, 4ug; Detection:17513-1-AP 1:1000) with HepG2 cells lysate 3600ug.
IHC staining of human lung cancer using 17513-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 17513-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 17513-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 17513-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 17513-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 17513-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 17513-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 17513-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 17513-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 17513-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 17513-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 17513-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). heat mediated antigen retrieved with .
IHC staining of human breast cancer using 17513-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). heat mediated antigen retrieved with .
IHC staining of human cervical cancer using 17513-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 17513-1-AP (KRT7-Specific antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervical cancer using 17513-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 17513-1-AP (KRT7-Specific antibody) at dilution of 1:50 (under 40x lens).
IF Staining of mouse liver using 17513-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 17513-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 17513-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 17513-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 17513-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 17513-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 7-specific (17513-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

Cytokeratin 7-specific Polyclonal antibody

Cytokeratin 7-specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 17513-1-AP

Publications(52)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Synonyms

Cytokeratin 7, KRT7, Cytokeratin-7, Cytokeratin,Keratin, CK-7

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (52)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, HeLa cells, T-47D cells, mouse bladder tissue, mouse lung tissue, rat bladder tissue, rat lung tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human lung cancer tissue, human bowen disease, human breast cancer tissue, human cervical cancer tissue, human kidney tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse liver tissue
Positive IF/ICC detected in	HeLa cells, HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:10000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 6 publications below
IHC	See 27 publications below
IF	See 24 publications below

Product Information

17513-1-AP targets Cytokeratin 7-specific in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	keratin 7
Calculated molecular weight	51 kDa
Observed molecular weight	51 kDa
GenBank accession number	NM_005556
Gene Symbol	Cytokeratin 7
Gene ID (NCBI)	3855
RRID	AB_2134468
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08729
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. This antibody is specifically against KRT7.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 7-specific antibody 17513-1-AP	Download protocol
IHC protocol for Cytokeratin 7-specific antibody 17513-1-AP	Download protocol
IF protocol for Cytokeratin 7-specific antibody 17513-1-AP	Download protocol
IP protocol for Cytokeratin 7-specific antibody 17513-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	IF	Transl Res SERPINA5 may promote the development of preeclampsia by disruption of the uPA/uPAR pathway. Authors - Yan Long View Article
human	IHC	Clin Cancer Res Integrative Genomic Analysis of Gemcitabine Resistance in Pancreatic Cancer by Patient-derived Xenograft Models. Authors - Gang Yang View Article
mouse	IHC	Angiogenesis A novel lymphatic pattern promotes metastasis of cervical cancer in a hypoxic tumour-associated macrophage-dependent manner. Authors - Xiao-Jing Chen View Article
rat	IHC,IF	Proc Natl Acad Sci U S A Up-regulation of CD81 inhibits cytotrophoblast invasion and mediates maternal endothelial cell dysfunction in preeclampsia. Authors - Li Shen View Article
mouse	IF	Cell Prolif Insufficient GDF15 expression predisposes women to unexplained recurrent pregnancy loss by impairing extravillous trophoblast invasion Authors - Chunzi Lyu View Article
mouse	IHC	Oncogene USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model. Authors - Juntae Kwon View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17513-1-AP

WB analysis using 17513-1-AP

WB analysis of T-47D using 17513-1-AP

WB analysis of HeLa using 17513-1-AP

IP experiment of HepG2 using 17513-1-AP

IHC staining of human lung cancer using 17513-1-AP

IHC staining of human lung cancer using 17513-1-AP

IHC staining of human lung cancer using 17513-1-AP

IHC staining of human bowen disease using 17513-1-AP

IHC staining of human bowen disease using 17513-1-AP

IHC staining of human kidney using 17513-1-AP

IHC staining of human kidney using 17513-1-AP

IHC staining of human ovary tumor using 17513-1-AP

IHC staining of human ovary tumor using 17513-1-AP

IHC staining of human kidney using 17513-1-AP

IHC staining of human kidney using 17513-1-AP

IHC staining of human breast cancer using 17513-1-AP

IHC staining of human breast cancer using 17513-1-AP

IHC staining of human cervical cancer using 17513-1-AP

IHC staining of human cervical cancer using 17513-1-AP

IF Staining of mouse liver using 17513-1-AP

IF Staining of HepG2 using 17513-1-AP

IF Staining of HepG2 using 17513-1-AP

IF Staining of HepG2 using 17513-1-AP

IF Staining of HeLa using 17513-1-AP

FC experiment of HeLa using 17513-1-AP

Cytokeratin 7-specific Polyclonal antibody

Cytokeratin 7-specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SERPINA5 may promote the development of preeclampsia by disruption of the uPA/uPAR pathway.

Integrative Genomic Analysis of Gemcitabine Resistance in Pancreatic Cancer by Patient-derived Xenograft Models.

A novel lymphatic pattern promotes metastasis of cervical cancer in a hypoxic tumour-associated macrophage-dependent manner.

Up-regulation of CD81 inhibits cytotrophoblast invasion and mediates maternal endothelial cell dysfunction in preeclampsia.

Insufficient GDF15 expression predisposes women to unexplained recurrent pregnancy loss by impairing extravillous trophoblast invasion

USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model.