Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, A549 cells, C6 cells, HeLa cells, MCF-7 cells |
| Positive IHC detected in | human stomach tissue, human colon, human bowen disease, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 9 publications below |
| IHC | See 11 publications below |
| IF | See 9 publications below |
Product Information
17514-1-AP targets Cytokeratin 8 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | keratin 8 |
| Calculated molecular weight | 54 kDa |
| Observed molecular weight | 54 kDa |
| GenBank accession number | NM_002273 |
| Gene Symbol | Cytokeratin 8 |
| Gene ID (NCBI) | 3856 |
| RRID | AB_2134726 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05787 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo. This antibody is specifically against KRT8.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 8 antibody 17514-1-AP | Download protocol |
| IHC protocol for Cytokeratin 8 antibody 17514-1-AP | Download protocol |
| IF protocol for Cytokeratin 8 antibody 17514-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer | ||
Clin Transl Med Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma. | ||
Sci Rep Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators. | ||
iScience Cysteine Dioxygenase Regulates the Epithelial Morphogenesis of Mammary Gland via Cysteine Sulfinic Acid. | ||
Regen Ther Dendrobium officinale polysaccharide promotes angiogenesis as well as follicle regeneration and hair growth through activation of the WNT signaling pathway | ||
Front Med (Lausanne) An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury. |