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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 8 Polyclonal antibody

Cytokeratin 8 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 17514-1-AP

Synonyms

KRT8, Cytokeratin-8, Cytokeratin,Keratin, CK-8, CK8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 8 Polyclonal antibody (17514-1-AP)

    WB analysis using 17514-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using Cytokeratin 8 Polyclonal antibody (17514-1-AP)

    WB analysis of C6 using 17514-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using Cytokeratin 8 Polyclonal antibody (17514-1-AP)

    WB analysis of A549 using 17514-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using Cytokeratin 8 Polyclonal antibody (17514-1-AP)

    WB analysis of A549 using 17514-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Cytokeratin 8 Polyclonal antibody (17514-1-AP)

    IHC staining of human stomach using 17514-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using Cytokeratin 8 Polyclonal antibody (17514-1-AP)

    IHC staining of human stomach using 17514-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using Cytokeratin 8 Polyclonal antibody (17514-1-AP)

    IHC staining of human bowen disease using 17514-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon using Cytokeratin 8 Polyclonal antibody (17514-1-AP)

    IHC staining of human colon using 17514-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon using Cytokeratin 8 Polyclonal antibody (17514-1-AP)

    IHC staining of human colon using 17514-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using Cytokeratin 8 Polyclonal antibody (17514-1-AP)

    IHC staining of human bowen disease using 17514-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using Cytokeratin 8 Polyclonal antibody (17514-1-AP)

    IHC staining of human bowen disease using 17514-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using Cytokeratin 8 Polyclonal antibody (17514-1-AP)

    IHC staining of human bowen disease using 17514-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Cytokeratin 8 Polyclonal antibody (17514-1-AP)

    IHC staining of human prostate cancer using 17514-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Cytokeratin 8 Polyclonal antibody (17514-1-AP)

    IHC staining of human prostate cancer using 17514-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using Cytokeratin 8 Polyclonal antibody (17514-1-AP)

    FC experiment of HeLa using 17514-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 8 (17514-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA431 cells, A549 cells, C6 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman stomach tissue, human colon, human bowen disease, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17514-1-AP targets Cytokeratin 8 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name keratin 8
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberNM_002273
Gene symbol Cytokeratin 8
Gene ID (NCBI) 3856
RRIDAB_2134726
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo. This antibody is specifically against KRT8.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 8 antibody 17514-1-APDownload protocol
IHC protocol for Cytokeratin 8 antibody 17514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer

Authors - Mengjie Li
View Article
humanWB

Clin Transl Med

Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.

Authors - An-Dong Liu
View Article
mouseIHC

Sci Rep

Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators.

Authors - Shu Wei
View Article
mouseWB,IF

iScience

Cysteine Dioxygenase Regulates the Epithelial Morphogenesis of Mammary Gland via Cysteine Sulfinic Acid.

Authors - Jianjun Zhao
View Article
mouseIF

Regen Ther

Dendrobium officinale polysaccharide promotes angiogenesis as well as follicle regeneration and hair growth through activation of the WNT signaling pathway

Authors - Yujin Zhang
View Article
mouseIHC

Front Med (Lausanne)

An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury.

Authors - Songyan Gao
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Emma (Verified Customer) (11-29-2021)

Works very well by IF on FFPE tissue. Used at 1:50 with a Tris-EDTA antigen retrieval.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Prostate
Cytokeratin 8 Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:17514-1-AP)
FH

Joleen (Verified Customer) (06-12-2019)

This antibody recognizes a band that matched with the expected molecular weight of KRT8.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
Cytokeratin 8 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:17514-1-AP)