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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using KTELC1 Polyclonal antibody (24409-1-AP)

    WB analysis of HepG2 using 24409-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24409-1-AP (KTELC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using KTELC1 Polyclonal antibody (24409-1-AP)

    WB analysis of HEK-293 using 24409-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24409-1-AP (KTELC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using KTELC1 Polyclonal antibody (24409-1-AP)

    WB analysis of HeLa using 24409-1-AP

    Hela cells were subjected to SDS PAGE followed by western blot with 24409-1-AP (KTELC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using KTELC1 Polyclonal antibody (24409-1-AP)

    IHC staining of human liver using 24409-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24409-1-AP (KTELC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using KTELC1 Polyclonal antibody (24409-1-AP)

    IHC staining of human liver using 24409-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24409-1-AP (KTELC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using KTELC1 Polyclonal antibody (24409-1-AP)

    IF Staining of C2C12 using 24409-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using KTELC1 antibody (24409-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using KTELC1 Polyclonal antibody (24409-1-AP)

    IF Staining of C2C12 using 24409-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using KTELC1 antibody (24409-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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KTELC1 Polyclonal antibody

KTELC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24409-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C3orf9, CAP10 like 46 kDa protein, CLP46, hCLP46, KDELCL1, KTELC1, MDS010, MDSRP, UNQ490/PRO1006

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, Hela cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24409-1-AP targets KTELC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KTELC1 fusion protein Ag19942 相同性解析による交差性が予測される生物種
Full Name KTEL (Lys-Tyr-Glu-Leu) containing 1
Calculated molecular weight 392 aa, 46 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC048810
Gene Symbol KTELC1
Gene ID (NCBI) 56983
RRIDAB_2879528
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NBL1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KTELC1 also termed as CLP46 is a glucosyltransferase, which implicates in Protein modification and glycosylation. KTELC1 encodes a 392 amino acids protein and its molecular weight is 46kda, but is exists glycosylation modification. KTELC1 is localized in endoplasmic reticulum lumen and abundantly expressed in liver, brain, heart, skeletal muscle, spleen, kidney, placenta, lung and peripheral blood leukocyte. KTELC1 may regulation in cell proliferation and Notch signal pathway.1

Protocols

Product Specific Protocols
WB protocol for KTELC1 antibody 24409-1-APDownload protocol
IHC protocol for KTELC1 antibody 24409-1-APDownload protocol
IF protocol for KTELC1 antibody 24409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols