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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KTI12 Polyclonal antibody (28237-1-AP)

    WB analysis using 28237-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28237-1-AP (KTI12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using KTI12 Polyclonal antibody (28237-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 28237-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 28237-1-AP (KTI12 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

KTI12 Polyclonal antibody

KTI12 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28237-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

TOT4, SBBI81, KTI 12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28237-1-AP targets KTI12 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KTI12 fusion protein Ag28201 相同性解析による交差性が予測される生物種
Full Name KTI12 homolog, chromatin associated (S. cerevisiae)
Calculated molecular weight 354 aa, 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC012173
Gene Symbol KTI12
Gene ID (NCBI) 112970
RRIDAB_2881094
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EK9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KTI12 antibody 28237-1-APDownload protocol
IHC protocol for KTI12 antibody 28237-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols