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  • Featured Product
  • KD/KO Validated

KU70,XRCC6 Polyclonal antibody

KU70,XRCC6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 10723-1-AP

Synonyms

KU70, XRCC6, CTC75, CTC box-binding factor 75 kDa subunit, ATP-dependent DNA helicase II 70 kDa subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    WB analysis using 10723-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    WB analysis using 10723-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    WB analysis of MCF-7 using 10723-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    WB analysis of A431 using 10723-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    IP experiment of HeLa using 10723-1-AP

    IP result of anti-KU70,XRCC6 (IP:10723-1-AP, 3ug; Detection:10723-1-AP 1:1000) with HeLa cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    IHC staining of human colon cancer using 10723-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    IHC staining of human lung cancer using 10723-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    IHC staining of human renal cell carcinoma using 10723-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    IHC staining of human renal cell carcinoma using 10723-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    IHC staining of human lung cancer using 10723-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    IHC staining of mouse testis using 10723-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    IHC staining of mouse testis using 10723-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    IHC staining of human colon cancer using 10723-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    IHC staining of human colon cancer using 10723-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    IHC staining of mouse kidney using 10723-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    IHC staining of mouse kidney using 10723-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    IHC staining of human colon cancer using 10723-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    IF Staining of HepG2 using 10723-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KU70 antibody (10723-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KU70,XRCC6 Polyclonal antibody (10723-1-AP)

    IF Staining of HepG2 using 10723-1-AP

    Immunofluorescent analysis of HepG2 cells, using KU70,XRCC6 antibody 10723-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inA431 cells, MCF-7 cells, mouse colon tissue, A549 cells, Hela cells, mouse spleen tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman renal cell carcinoma tissue, human colon cancer tissue, human lung cancer tissue, mouse kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10723-1-AP targets KU70,XRCC6 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KU70,XRCC6 fusion protein Ag1043 相同性解析による交差性が予測される生物種
Full Name X-ray repair complementing defective repair in Chinese hamster cells 6
Calculated molecular weight 75 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC008343
Gene symbol KU70
Gene ID (NCBI) 2547
RRIDAB_2218756
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.

Protocols

Product Specific Protocols
WB protocol for KU70,XRCC6 antibody 10723-1-APDownload protocol
IHC protocol for KU70,XRCC6 antibody 10723-1-APDownload protocol
IF protocol for KU70,XRCC6 antibody 10723-1-APDownload protocol
IP protocol for KU70,XRCC6 antibody 10723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Cell

A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis

Authors - Kaiwen Bao
View Article
humanWB,IF

Oncogene

UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy.

Authors - L Hu
View Article
humanWB

Phytomedicine

Kaempferol inhibits non-homologous end joining repair via regulating Ku80 stability in glioma cancer

Authors - Meiyang Chen
View Article
humanWB

J Mater Chem B

Hollow PtCo alloy nanospheres as a high-Z and oxygen generating nanozyme for radiotherapy enhancement in non-small cell lung cancer

Authors - Shuying Li
View Article
humanWB

FEBS J

Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy

Authors - Yan Wei
View Article
ratWB

FASEB J

Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats.

Authors - Catherine E Aiken
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Jonathan (Verified Customer) (02-03-2019)

Worked well for Western Blotting with little background.

  • Applications: Western Blot,