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Species Reactivity

Species Reactivity

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Conjugate

KU70,XRCC6 Monoclonal antibody, PBS Only

KU70,XRCC6 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C12D9

Cat no : 66607-1-PBS

Synonyms

KU70, XRCC6, CTC box-binding factor 75 kDa subunit, ATP-dependent DNA helicase II 70 kDa subunit, ATP-dependent DNA helicase 2 subunit 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KU70,XRCC6 Monoclonal antibody (66607-1-Ig)

    WB analysis using 66607-1-Ig (same clone as 66607-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KU70,XRCC6 Monoclonal antibody (66607-1-Ig)

    WB analysis using 66607-1-Ig (same clone as 66607-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using KU70,XRCC6 Monoclonal antibody (66607-1-Ig)

    WB analysis of HSC-T6 using 66607-1-Ig (same clone as 66607-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using KU70,XRCC6 Monoclonal antibody (66607-1-Ig)

    WB analysis of NIH/3T3 using 66607-1-Ig (same clone as 66607-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using KU70,XRCC6 Monoclonal antibody (66607-1-Ig)

    WB analysis of RAW 264.7 using 66607-1-Ig (same clone as 66607-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KU70,XRCC6 Monoclonal antibody (66607-1-Ig)

    IHC staining of human lung cancer using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KU70,XRCC6 Monoclonal antibody (66607-1-Ig)

    IHC staining of human lung cancer using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KU70,XRCC6 Monoclonal antibody (66607-1-Ig)

    IHC staining of human breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KU70,XRCC6 Monoclonal antibody (66607-1-Ig)

    IHC staining of human breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KU70,XRCC6 Monoclonal antibody (66607-1-Ig)

    IHC staining of human breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KU70,XRCC6 Monoclonal antibody (66607-1-Ig)

    IHC staining of human colon cancer using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KU70,XRCC6 Monoclonal antibody (66607-1-Ig)

    IHC staining of human colon cancer using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KU70,XRCC6 Monoclonal antibody (66607-1-Ig)

    IHC staining of human breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using KU70,XRCC6 Monoclonal antibody (66607-1-Ig)

    IF Staining of human breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using KU70,XRCC6 Monoclonal antibody (66607-1-Ig)

    IF Staining of human breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KU70,XRCC6 Monoclonal antibody (66607-1-Ig)

    IF Staining of HepG2 using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KU70,XRCC6 antibody (66607-1-Ig, Clone: 3C12D9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KU70,XRCC6 Monoclonal antibody (66607-1-Ig)

    IF Staining of HeLa using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KU70,XRCC6 antibody (66607-1-Ig, Clone: 3C12D9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using KU70,XRCC6 Monoclonal antibody (66607-1-Ig)

    FC experiment of HepG2 using 66607-1-Ig (same clone as 66607-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human KU70,XRCC6 (66607-1-Ig, Clone:3C12D9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66607-1-PBS targets KU70,XRCC6 in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KU70,XRCC6 fusion protein Ag21301 相同性解析による交差性が予測される生物種
Full Name X-ray repair complementing defective repair in Chinese hamster cells 6
Calculated molecular weight 75 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC008343
Gene symbol KU70
Gene ID (NCBI) 2547
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.