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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using Kallikrein 11 Polyclonal antibody (12698-1-AP)

    WB analysis of MCF-7 using 12698-1-AP (same clone as 12698-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12698-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Kallikrein 11 Polyclonal antibody (12698-1-AP)

    WB analysis of HeLa using 12698-1-AP (same clone as 12698-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12698-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using Kallikrein 11 Polyclonal antibody (12698-1-AP)

    WB analysis of PC-3 using 12698-1-AP (same clone as 12698-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12698-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using Kallikrein 11 Polyclonal antibody (12698-1-AP)

    WB analysis of Y79 using 12698-1-AP (same clone as 12698-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12698-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Kallikrein 11 Polyclonal antibody (12698-1-AP)

    IHC staining of human prostate cancer using 12698-1-AP (same clone as 12698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12698-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Kallikrein 11 Polyclonal antibody (12698-1-AP)

    IHC staining of human brain using 12698-1-AP (same clone as 12698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12698-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Kallikrein 11 Polyclonal antibody (12698-1-AP)

    IHC staining of human brain using 12698-1-AP (same clone as 12698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12698-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Kallikrein 11 Polyclonal antibody (12698-1-AP)

    IHC staining of human prostate cancer using 12698-1-AP (same clone as 12698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12698-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Kallikrein 11 Polyclonal antibody (12698-1-AP)

    IHC staining of human prostate cancer using 12698-1-AP (same clone as 12698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12698-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using Kallikrein 11 Polyclonal antibody (12698-1-AP)

    IF Staining of PC-3 using 12698-1-AP (same clone as 12698-1-PBS)

    Immunofluorescent analysis of PC-3 cells using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12698-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12698-1-PBS targets Kallikrein 11 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kallikrein 11 fusion protein Ag3427 相同性解析による交差性が予測される生物種
Full Name kallikrein-related peptidase 11
Calculated molecular weight 250 aa, 27 kDa
Observed molecular weight 30-40 kDa
GenBank accession numberBC022068
Gene Symbol KLK11
Gene ID (NCBI) 11012
RRIDAB_2877876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBX7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.