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  • Featured Product
  • KD/KO Validated

Keratin 81 Polyclonal antibody, PBS Only

Keratin 81 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11342-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Keratin 81 Polyclonal antibody (11342-1-AP)

    WB analysis using 11342-1-AP (same clone as 11342-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Keratin 81 Polyclonal antibody (11342-1-AP)

    WB analysis using 11342-1-AP (same clone as 11342-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Keratin 81 Polyclonal antibody (11342-1-AP)

    WB analysis of HEK-293 using 11342-1-AP (same clone as 11342-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using Keratin 81 Polyclonal antibody (11342-1-AP)

    WB analysis of mouse thymus using 11342-1-AP (same clone as 11342-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using Keratin 81 Polyclonal antibody (11342-1-AP)

    WB analysis of human spleen using 11342-1-AP (same clone as 11342-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using Keratin 81 Polyclonal antibody (11342-1-AP)

    IP experiment of HEK-293 using 11342-1-AP (same clone as 11342-1-PBS)

    IP result of anti-KRT81 (IP:11342-1-AP, 4ug; Detection:11342-1-AP 1:300) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Keratin 81 Polyclonal antibody (11342-1-AP)

    IHC staining of human colon cancer using 11342-1-AP (same clone as 11342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11342-1-AP (KRT81 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Keratin 81 Polyclonal antibody (11342-1-AP)

    IF Staining of HeLa using 11342-1-AP (same clone as 11342-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KRT81 antibody (11342-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11342-1-PBS targets Keratin 81 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Keratin 81 fusion protein Ag1890 相同性解析による交差性が予測される生物種
Full Name keratin 81
Calculated molecular weight 55 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC021241
Gene symbol KRT81
Gene ID (NCBI) 3887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT81 is a type II keratin.