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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Ki-67 Monoclonal antibody (66555-6-Ig)

    IHC staining of human tonsillitis using 66555-6-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Ki-67 Monoclonal antibody (66555-6-Ig)

    IHC staining of human tonsillitis using 66555-6-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using Ki-67 Monoclonal antibody (66555-6-Ig)

    IHC staining of human ovary cancer using 66555-6-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using Ki-67 Monoclonal antibody (66555-6-Ig)

    IHC staining of human ovary cancer using 66555-6-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using Ki-67 Monoclonal antibody (66555-6-Ig)

    IHC staining of human rectal cancer using 66555-6-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using Ki-67 Monoclonal antibody (66555-6-Ig)

    IHC staining of human rectal cancer using 66555-6-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Ki-67 Monoclonal antibody (66555-6-Ig)

    IF Staining of A431 using 66555-6-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Ki-67 antibody (66555-6-Ig, Clone: 1B9H2 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Ki-67 Monoclonal antibody (66555-6-Ig)

    IF Staining of HeLa using 66555-6-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Ki-67 antibody (66555-6-Ig, Clone: 1B9H2 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Ki-67 Monoclonal antibody

Ki-67 Monoclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 66555-6-Ig
Clone No.1B9H2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human ovary cancer tissue, human rectal cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66555-6-Ig targets Ki-67 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Ki-67 fusion protein Ag26266 相同性解析による交差性が予測される生物種
Full Name antigen identified by monoclonal antibody Ki-67
Calculated molecular weight 359 kDa
GenBank accession numberNM_002417
Gene Symbol KI67
Gene ID (NCBI) 4288
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP46013
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

Protocols

Product Specific Protocols
IHC protocol for Ki-67 antibody 66555-6-IgDownload protocol
IF protocol for Ki-67 antibody 66555-6-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols