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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Ki-67 Recombinant antibody

Ki-67 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

241499E7

Cat no : 84192-4-RR

Synonyms

MKI67, KI67, Ki 67, Antigen KI-67, Antigen identified by monoclonal antibody Ki-67

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ki-67 Recombinant antibody (84192-4-RR)

    WB analysis using 84192-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84192-4-RR (Ki-67 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Ki-67 Recombinant antibody (84192-4-RR)

    IHC staining of human tonsillitis using 84192-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Ki-67 Recombinant antibody (84192-4-RR)

    IHC staining of human tonsillitis using 84192-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Ki-67 Recombinant antibody (84192-4-RR)

    IHC staining of human skin cancer using 84192-4-RR

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Ki-67 Recombinant antibody (84192-4-RR)

    IHC staining of human skin cancer using 84192-4-RR

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of Human Spleen tissue using Ki-67 Recombinant antibody (84192-4-RR)

    IHC staining of Human Spleen using 84192-4-RR

    Immunohistochemical analysis of paraffin-embedded Human Spleen tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of Human Spleen tissue using Ki-67 Recombinant antibody (84192-4-RR)

    IHC staining of Human Spleen using 84192-4-RR

    Immunohistochemical analysis of paraffin-embedded Human Spleen tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of Human renal cancer using Ki-67 Recombinant antibody (84192-4-RR)

    IHC staining of Human renal cancer using 84192-4-RR

    Immunohistochemical analysis of paraffin-embedded Human renal cancer slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of Human renal cancer using Ki-67 Recombinant antibody (84192-4-RR)

    IHC staining of Human renal cancer using 84192-4-RR

    Immunohistochemical analysis of paraffin-embedded Human renal cancer slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using Ki-67 Recombinant antibody (84192-4-RR)

    IHC staining of human malignant melanoma using 84192-4-RR

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using Ki-67 Recombinant antibody (84192-4-RR)

    IHC staining of human malignant melanoma using 84192-4-RR

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Ki-67 Recombinant antibody (84192-4-RR)

    IHC staining of human prostate cancer using 84192-4-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Ki-67 Recombinant antibody (84192-4-RR)

    IHC staining of human prostate cancer using 84192-4-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human Breast cancer tissue using Ki-67 Recombinant antibody (84192-4-RR)

    IHC staining of human Breast cancer using 84192-4-RR

    Immunohistochemical analysis of paraffin-embedded human Breast cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human Breast cancer tissue using Ki-67 Recombinant antibody (84192-4-RR)

    IHC staining of human Breast cancer using 84192-4-RR

    Immunohistochemical analysis of paraffin-embedded human Breast cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using Ki-67 Recombinant antibody (84192-4-RR)

    IF Staining of human lung cancer using 84192-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1/CD54 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using Ki-67 Recombinant antibody (84192-4-RR)

    IF Staining of human lung cancer using 84192-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human thyroid cancer tissue using Ki-67 Recombinant antibody (84192-4-RR)

    IF Staining of human thyroid cancer using 84192-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human thyroid cancer tissue using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human thyroid cancer tissue using Ki-67 Recombinant antibody (84192-4-RR)

    IF Staining of human thyroid cancer using 84192-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human thyroid cancer tissue using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1/CD54 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Ki-67 Recombinant antibody (84192-4-RR)

    IF Staining of HeLa using 84192-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Ki-67 Recombinant antibody (84192-4-RR)

    IF Staining of A549 using 84192-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Ki-67 Recombinant antibody (84192-4-RR)

    IF Staining of A431 using 84192-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Ki-67 Recombinant antibody (84192-4-RR)

    IF Staining of HepG2 using 84192-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using Ki-67 Recombinant antibody (84192-4-RR)

    IF Staining of U2OS using 84192-4-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Ki-67 Recombinant antibody (84192-4-RR)

    IF Staining of MCF-7 using 84192-4-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, MCF-7 cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue, human Breast cancer tissue, human malignant melanoma tissue, human prostate cancer tissue, Human renal cancer, human skin cancer tissue, Human Spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung cancer tissue, human thyroid cancer tissue
Positive IF/ICC detected inHeLa cells, MCF-7 cells, U2OS cells, HepG2 cells, A431 cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84192-4-RR targets Ki-67 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name antigen identified by monoclonal antibody Ki-67
Calculated molecular weight 359 kDa
GenBank accession numberNM_002417
Gene symbol KI67
Gene ID (NCBI) 4288
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

Protocols

Product Specific Protocols
WB protocol for Ki-67 antibody 84192-4-RRDownload protocol
IHC protocol for Ki-67 antibody 84192-4-RRDownload protocol
IF protocol for Ki-67 antibody 84192-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols