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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human Ki-67 Rabbit Recombinant Antibody, PBS Only

Ki-67 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

241499B1

Cat no : 98143-2-PBS

Synonyms

MKI67, KI67, Ki 67, Antigen KI-67, Antigen identified by monoclonal antibody Ki-67

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human Ki-67 Rabbit Recombinant Antibody (98143-2-RR)

    FC experiment of human PBMCs using 98143-2-RR (same clone as 98143-2-PBS)

    1x10^6 untreated or PHA-treated human PBMCs were intracellularly stained with 0.25 ug Ki-67 Recombinant Antibody (98143-2-RR, Clone: 241499B1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98143-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human Ki-67 Rabbit Recombinant Antibody (98143-2-RR)

    FC experiment of human PBMCs using 98143-2-RR (same clone as 98143-2-PBS)

    1x10^6 PHA treated human PBMCs were intracellularly stained with 0.25 ug Ki-67 Recombinant Antibody (98143-2-RR, Clone: 241499B1) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Ki-67 Recombinant Antibody (98143-2-RR, Clone:241499B1) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98143-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98143-2-PBS targets Ki-67 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name antigen identified by monoclonal antibody Ki-67
Calculated molecular weight 359 kDa
GenBank accession numberNM_002417
Gene symbol KI67
Gene ID (NCBI) 4288
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.