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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Mouse Ki-67 Rabbit Recombinant Antibody, PBS Only

Ki-67 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

241768E2

Cat no : 98224-1-PBS

Synonyms

Mki67, Ki67, Ki 67, Proliferation related Ki 67 antigen, 241768E2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of C57BL/6 mouse splenocytes using Anti-Mouse Ki-67 Rabbit Recombinant Antibody (98224-1-RR)

    FC experiment of C57BL/6 mouse splenocytes using 98224-1-RR (same clone as 98224-1-PBS)

    1x10^6 untreated or Con A treated C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse Ki-67 Rabbit Recombinant Antibody (98224-1-RR, Clone: 241768E2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98224-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of C57BL/6 mouse splenocytes using Anti-Mouse Ki-67 Rabbit Recombinant Antibody (98224-1-RR)

    FC experiment of C57BL/6 mouse splenocytes using 98224-1-RR (same clone as 98224-1-PBS)

    1x10^6 Con A treated C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse Ki-67 Rabbit Recombinant Antibody (98224-1-RR, Clone: 241768E2) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse Ki-67 Rabbit Recombinant Antibody (98224-1-RR, Clone: 241768E2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98224-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98224-1-PBS targets Ki-67 in FC (Intra) applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name antigen identified by monoclonal antibody Ki 67
Calculated molecular weight351kDa
GenBank accession numberNM_001081117.2
Gene symbol ki67
Gene ID (NCBI) 17345
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki-67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.