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  • Featured Product
  • KD/KO Validated

Kindlin 1 Polyclonal antibody, PBS Only

Kindlin 1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22215-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Kindlin 1 Polyclonal antibody (22215-1-AP)

    WB analysis using 22215-1-AP (same clone as 22215-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22215-1-AP (Kindlin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using Kindlin 1 Polyclonal antibody (22215-1-AP)

    WB analysis of COLO 320 using 22215-1-AP (same clone as 22215-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 22215-1-AP (FERMT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Kindlin 1 Polyclonal antibody (22215-1-AP)

    WB analysis of HEK-293 using 22215-1-AP (same clone as 22215-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22215-1-AP (FERMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Kindlin 1 Polyclonal antibody (22215-1-AP)

    IHC staining of human colon cancer using 22215-1-AP (same clone as 22215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (Kindlin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Kindlin 1 Polyclonal antibody (22215-1-AP)

    IHC staining of human colon cancer using 22215-1-AP (same clone as 22215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (Kindlin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Kindlin 1 Polyclonal antibody (22215-1-AP)

    IHC staining of human colon cancer using 22215-1-AP (same clone as 22215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (FERMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Kindlin 1 Polyclonal antibody (22215-1-AP)

    IHC staining of human pancreas using 22215-1-AP (same clone as 22215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Kindlin 1 Polyclonal antibody (22215-1-AP)

    IHC staining of human pancreas using 22215-1-AP (same clone as 22215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Kindlin 1 Polyclonal antibody (22215-1-AP)

    IHC staining of human colon cancer using 22215-1-AP (same clone as 22215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (FERMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Kindlin 1 Polyclonal antibody (22215-1-AP)

    IHC staining of human pancreas cancer using 22215-1-AP (same clone as 22215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Kindlin 1 Polyclonal antibody (22215-1-AP)

    IHC staining of human pancreas cancer using 22215-1-AP (same clone as 22215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Kindlin 1 Polyclonal antibody (22215-1-AP)

    IF Staining of HEK-293 using 22215-1-AP (same clone as 22215-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 22215-1-AP (FERMT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22215-1-PBS targets Kindlin 1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kindlin 1 fusion protein Ag17543 相同性解析による交差性が予測される生物種
Full Name fermitin family homolog 1 (Drosophila)
Calculated molecular weight 677 aa, 77 kDa
Observed molecular weight 70-77 kDa
GenBank accession numberBC035882
Gene symbol Kindlin 1
Gene ID (NCBI) 55612
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Kindlin-1 is a FERM domain containing adaptor protein that is found predominantly at cell-extracellular matrix adhesions where it binds to β-integrin subunits and is required for integrin activation. Loss of function mutations in the FERMT1 gene which encodes Kindlin-1 leads to the development of Kindler Syndrome (KS) an autosomal recessive skin disorder characterized by skin blistering, photosensitivity, and predisposition to aggressive squamous cell carcinoma (SCC).