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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Kindlin 2 Polyclonal antibody

Kindlin 2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29911-1-AP

Synonyms

FERMT2, FERMT 2, mig 2, Kindlin-2, kindlin2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Kindlin 2 Polyclonal antibody (29911-1-AP)

    WB analysis using 29911-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29911-1-AP (Kindlin 2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human normal colon using Kindlin 2 Polyclonal antibody (29911-1-AP)

    IHC staining of human normal colon using 29911-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 29911-1-AP (Kindlin 2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human normal colon using Kindlin 2 Polyclonal antibody (29911-1-AP)

    IHC staining of human normal colon using 29911-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 29911-1-AP (Kindlin 2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Kindlin 2 Polyclonal antibody (29911-1-AP)

    IHC staining of human lung cancer using 29911-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29911-1-AP (Kindlin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29911-1-AP targets Kindlin 2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kindlin 2 fusion protein Ag31795 相同性解析による交差性が予測される生物種
Full Name fermitin family homolog 2 (Drosophila)
Calculated molecular weight 680 aa, 78 kDa
Observed molecular weight78 kDa
GenBank accession numberBC017327
Gene symbol Kindlin 2
Gene ID (NCBI) 10979
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FERMT2, also named as KIND2, MIG2, PLEKHC1, MIG-2 and Kindlin-2, belongs to the kindlin family. There are three FERMT2 isoforms that participate in the connection between extracellular matrix (ECM) adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. FERMT2 recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. Variable expression in human cancers and mesenchymal cancer invasion are now linked with FERMT2, which may plays important role in carcinormas development. Catalog#11453-1-AP is a rabbit polyclonal antibody against human FERMT2.

Protocols

Product Specific Protocols
WB protocol for Kindlin 2 antibody 29911-1-APDownload protocol
IHC protocol for Kindlin 2 antibody 29911-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols