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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Kindlin 3 Polyclonal antibody (18131-1-AP)

    WB analysis of Jurkat using 18131-1-AP (same clone as 18131-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Kindlin 3 Polyclonal antibody (18131-1-AP)

    WB analysis using 18131-1-AP (same clone as 18131-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18131-1-AP (Kindlin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Kindlin 3 Polyclonal antibody (18131-1-AP)

    WB analysis using 18131-1-AP (same clone as 18131-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18131-1-AP (Kindlin 3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using Kindlin 3 Polyclonal antibody (18131-1-AP)

    WB analysis of Raji using 18131-1-AP (same clone as 18131-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using Kindlin 3 Polyclonal antibody (18131-1-AP)

    WB analysis of Jurkat using 18131-1-AP (same clone as 18131-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using Kindlin 3 Polyclonal antibody (18131-1-AP)

    WB analysis of Raji using 18131-1-AP (same clone as 18131-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using Kindlin 3 Polyclonal antibody (18131-1-AP)

    WB analysis of Raji using 18131-1-AP (same clone as 18131-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using Kindlin 3 Polyclonal antibody (18131-1-AP)

    WB analysis of Raji using 18131-1-AP (same clone as 18131-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Kindlin 3 Polyclonal antibody (18131-1-AP)

    IHC staining of human lymphoma using 18131-1-AP (same clone as 18131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 18131-1-AP (FERMT3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Kindlin 3 Polyclonal antibody (18131-1-AP)

    IHC staining of human lymphoma using 18131-1-AP (same clone as 18131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 18131-1-AP (FERMT3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Kindlin 3 Polyclonal antibody (18131-1-AP)

    IF Staining of HeLa using 18131-1-AP (same clone as 18131-1-PBS)

    Immunofluorescent analysis of HeLa cells using 18131-1-AP (FERMT3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

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Kindlin 3 Polyclonal antibody, PBS Only

Kindlin 3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18131-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18131-1-PBS targets Kindlin 3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kindlin 3 fusion protein Ag12713 相同性解析による交差性が予測される生物種
Full Name fermitin family homolog 3 (Drosophila)
Calculated molecular weight 76 kDa
Observed molecular weight 72-76 kDa
GenBank accession numberBC004347
Gene Symbol Kindlin 3/FERMT3
Gene ID (NCBI) 83706
RRIDAB_2103056
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86UX7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Kindlin-3 (FERMT-3) is known to be central in hemostasis and thrombosis control and its deficiency disrupts platelet aggregation and causes Leukocyte Adhesion Deficiency disease. Point mutations in the kindlin-3 gene have been identified in humans with a rare inherited Integrin Activation efficiency Disease (IADD), also designated as Leukocyte Adhesion Deficiency syndrome (LAD-III or LADI variant). Kindlin-3 expression is restricted to cells of hematopoietic origin.