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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Kininogen 1 Polyclonal antibody (27477-1-AP)

    WB analysis using 27477-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27477-1-AP (Kininogen 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Kininogen 1 Polyclonal antibody (27477-1-AP)

    WB analysis of HeLa using 27477-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27477-1-AP (Kininogen 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Kininogen 1 Polyclonal antibody (27477-1-AP)

    IHC staining of human kidney using 27477-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27477-1-AP (Kininogen 1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Kininogen 1 Polyclonal antibody (27477-1-AP)

    IHC staining of human kidney using 27477-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27477-1-AP (Kininogen 1 antibody) at dilution of 1:200 (under 40x lens).

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Kininogen 1 Polyclonal antibody

Kininogen 1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27477-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

BDK, Fitzgerald factor, HMWK, Ile Ser Bradykinin Bradykinin, Kallidin I Lysyl bradykinin, kininogen 1, KNG, KNG1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA2780 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27477-1-AP targets Kininogen 1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kininogen 1 fusion protein Ag26467 相同性解析による交差性が予測される生物種
Full Name kininogen 1
Calculated molecular weight 644 aa, 72 kDa
Observed molecular weight 70-72 kDa
GenBank accession numberBC060039
Gene Symbol Kininogen 1
Gene ID (NCBI) 3827
RRIDAB_2880882
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01042
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This gene is translated into High-molecular weight kininogen (HMWK) and low-molecular weight kininogen (LMWK) after alternative splicing. HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.

Protocols

Product Specific Protocols
WB protocol for Kininogen 1 antibody 27477-1-APDownload protocol
IHC protocol for Kininogen 1 antibody 27477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols