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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Kininogen-1 Recombinant antibody, PBS Only

Kininogen-1 Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241397C11

Cat no : 84175-5-PBS

Synonyms

KNG1, HMWK, Fitzgerald factor, Bradykinin, BDK

Validation Data Gallery

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  • Western Blot (WB) analysis of rat plasma using Kininogen-1 Recombinant antibody (84175-5-RR)

    WB analysis of rat plasma using 84175-5-RR (same clone as 84175-5-PBS)

    Rat plasma was subjected to SDS PAGE followed by western blot with 84175-5-RR (Kininogen-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84175-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human plasma using Kininogen-1 Recombinant antibody (84175-5-RR)

    WB analysis of human plasma using 84175-5-RR (same clone as 84175-5-PBS)

    Human plasma was subjected to SDS PAGE followed by western blot with 84175-5-RR (Kininogen-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84175-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HT-29 cells using Kininogen-1 Recombinant antibody (84175-5-RR)

    FC experiment of HT-29 using 84175-5-RR (same clone as 84175-5-PBS)

    1x10^6 HT-29 cells were intracellularly stained with 0.25 ug Kininogen-1 Recombinant antibody (84175-5-RR, Clone:241397C11) (red), or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84175-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84175-5-RR

    Affinity and Kinetic Characterization of 84175-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84175-5-RR against Human Kininogen-1 were performed. The affinity constant is 0.182 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84175-5-PBS is Kininogen-1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name kininogen 1
Calculated molecular weight72kDa
Observed molecular weight 48 kDa
GenBank accession numberNM_001102416.3
Gene symbol Kininogen 1
Gene ID (NCBI) 3827
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This KNG1 gene contains 10 exons and encodes two proteins through alternative splicing: high molecular weight kininogen (HMWK) (72kDa) and low molecular weight kininogen (LMWK) (48kDa) (PMID: 37442062). HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.