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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Kv4.2 Recombinant antibody (85133-3-RR)

    WB analysis using 85133-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85133-3-RR (KCND2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using Kv4.2 Recombinant antibody (85133-3-RR)

    WB analysis of DU 145 using 85133-3-RR

    DU 145 cells were subjected to SDS PAGE followed by western blot with 85133-3-RR (KCND2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Kv4.2 Recombinant antibody (85133-3-RR)

    IHC staining of mouse cerebellum using 85133-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85133-3-RR (Kv4.2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Kv4.2 Recombinant antibody (85133-3-RR)

    IHC staining of mouse cerebellum using 85133-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85133-3-RR (Kv4.2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Kv4.2 Recombinant antibody (85133-3-RR)

    IF Staining of mouse brain using 85133-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Kv4.2 antibody (85133-3-RR, Clone: 242754F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85133-3-RR

    Affinity and Kinetic Characterization of 85133-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85133-3-RR against Human Kv4.2 were performed. The affinity constant is below 1 pM.

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Kv4.2 Recombinant antibody

Kv4.2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 85133-3-RR
Clone No.242754F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

KCND2, 242754F4, Voltage-gated potassium channel subunit Kv4.2, RK5, Potassium voltage-gated channel subfamily D member 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, DU 145 cells, A549 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85133-3-RR targets Kv4.2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Kv4.2 fusion protein Ag15879 相同性解析による交差性が予測される生物種
Full Name potassium voltage-gated channel, Shal-related subfamily, member 2
Calculated molecular weight 630 aa, 71 kDa
Observed molecular weight75 kDa
GenBank accession numberBC110449
Gene Symbol KCND2
Gene ID (NCBI) 3751
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NZV8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Voltage-gated potassium or Kv channels, specifically those mediating low threshold, rapidly inactivating Ito and IA currents, are known to regulate cardiac and neuronal membrane excitability, respectively (PMID: 12829703). Voltage-gated potassium channel subunit Kv4.2, encoded by the KCND2 gene, belongs to the potassium channel family and D (Shal) subfamily. It is a pore-forming alpha subunit of voltage-gated rapidly inactivating A-type potassium channels. Kv4.2 is highly expressed in the brain (PMID: 10551270). It is a major constituent of A-type potassium currents and a key regulator of neuronal membrane excitability (PMID: 22539834).

Protocols

Product Specific Protocols
WB protocol for Kv4.2 antibody 85133-3-RRDownload protocol
IHC protocol for Kv4.2 antibody 85133-3-RRDownload protocol
IF protocol for Kv4.2 antibody 85133-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols