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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

L-Plastin Polyclonal antibody

L-Plastin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55208-1-AP

Synonyms

L PLASTIN, LCP 1, LCP1, LPL, L-Plastin, Lymphocyte cytosolic protein 1, Plastin 2, PLS2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using L-Plastin Polyclonal antibody (55208-1-AP)

    WB analysis using 55208-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55208-1-AP (L-Plastin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using L-Plastin Polyclonal antibody (55208-1-AP)

    IHC staining of human colon cancer using 55208-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55208-1-AP (L-Plastin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using L-Plastin Polyclonal antibody (55208-1-AP)

    IF Staining of A431 using 55208-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 55208-1-AP (L-Plastin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, Ramos cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55208-1-AP targets L-Plastin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name lymphocyte cytosolic protein 1 (L-plastin)
Calculated molecular weight 70 kDa
Observed molecular weight 64-70 kDa
GenBank accession numberNM_002298
Gene symbol L-Plastin
Gene ID (NCBI) 3936
RRIDAB_2881288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for L-Plastin antibody 55208-1-APDownload protocol
IHC protocol for L-Plastin antibody 55208-1-APDownload protocol
IF protocol for L-Plastin antibody 55208-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols