• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of MCF-7 cells using L-VEGFA Polyclonal antibody (22341-1-AP)

    WB analysis of MCF-7 using 22341-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 22341-1-AP (L-VEGFA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using L-VEGFA Polyclonal antibody (22341-1-AP)

    WB analysis using 22341-1-AP

    bEnd.3 cells were subjected to SDS PAGE followed by western blot with 22341-1-AP (L-VEGFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using L-VEGFA Polyclonal antibody (22341-1-AP)

    WB analysis of SH-SY5Y using 22341-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22341-1-AP (L-VEGFA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using L-VEGFA Polyclonal antibody (22341-1-AP)

    IHC staining of human liver cancer using 22341-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using L-VEGFA Polyclonal antibody (22341-1-AP)

    IHC staining of human liver cancer using 22341-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using L-VEGFA Polyclonal antibody (22341-1-AP)

    IHC staining of human colon cancer using 22341-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using L-VEGFA Polyclonal antibody (22341-1-AP)

    IHC staining of human colon cancer using 22341-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using L-VEGFA Polyclonal antibody (22341-1-AP)

    IHC staining of human liver cancer using 22341-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using L-VEGFA Polyclonal antibody (22341-1-AP)

    IHC staining of human liver cancer using 22341-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

L-VEGFA Polyclonal antibody

L-VEGFA Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22341-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

VEGF, VEGFA, Vascular permeability factor, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, bEnd.3 cells, SH-SY5Y cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

22341-1-AP targets L-VEGFA in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen L-VEGFA fusion protein Ag17831 相同性解析による交差性が予測される生物種
Full Name vascular endothelial growth factor A
Calculated molecular weight 412 aa, 46 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC065522
Gene Symbol VEGFA
Gene ID (NCBI) 7422
RRIDAB_2879080
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15692
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1).

Protocols

Product Specific Protocols
WB protocol for L-VEGFA antibody 22341-1-APDownload protocol
IHC protocol for L-VEGFA antibody 22341-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Carcinog

PMID: 28833603

Authors - Hui-Hui Zhang
View Article
mouseWB

Life Sci

Circular RNA COL1A2 promotes angiogenesis via regulating miR-29b/VEGF axis in diabetic retinopathy.

Authors - Jing Zou
View Article

Tumour Biol

Evodiamine exerts anti-tumor effects against hepatocellular carcinoma through inhibiting β-catenin-mediated angiogenesis.

Authors - Le Shi
View Article