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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

L-VOCC Polyclonal antibody, PBS Only

L-VOCC Polyclonal Antibody for IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 21774-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of mouse brain tissue using L-VOCC Polyclonal antibody (21774-1-AP)

    IHC staining of mouse brain using 21774-1-AP (same clone as 21774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21774-1-AP (L-VOCC antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using L-VOCC Polyclonal antibody (21774-1-AP)

    IHC staining of mouse brain using 21774-1-AP (same clone as 21774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21774-1-AP (L-VOCC antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using L-VOCC Polyclonal antibody (21774-1-AP)

    IHC staining of rat brain using 21774-1-AP (same clone as 21774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21774-1-AP (L-VOCC Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using L-VOCC Polyclonal antibody (21774-1-AP)

    IHC staining of rat brain using 21774-1-AP (same clone as 21774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21774-1-AP (L-VOCC Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using L-VOCC Polyclonal antibody (21774-1-AP)

    IHC staining of mouse liver using 21774-1-AP (same clone as 21774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver using 21774-1-AP (L-VOCC antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using L-VOCC Polyclonal antibody (21774-1-AP)

    IHC staining of mouse liver using 21774-1-AP (same clone as 21774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver using 21774-1-AP (L-VOCC antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using L-VOCC Polyclonal antibody (21774-1-AP)

    IF Staining of mouse brain using 21774-1-AP (same clone as 21774-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L-VOCC antibody (21774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using L-VOCC Polyclonal antibody (21774-1-AP)

    IF Staining of mouse brain using 21774-1-AP (same clone as 21774-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L-VOCC antibody (21774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using L-VOCC Polyclonal antibody (21774-1-AP)

    FC experiment of HeLa using 21774-1-AP (same clone as 21774-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human L-VOCC (21774-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21774-1-PBS targets L-VOCC in IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen L-VOCC fusion protein Ag16455 相同性解析による交差性が予測される生物種
Full Name calcium channel, voltage-dependent, L type, alpha 1C subunit
Calculated molecular weight 2135 aa, 239 kDa
GenBank accession numberBC146846
Gene symbol CACNA1C
Gene ID (NCBI) 775
RRIDAB_2878918
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.