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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CACNA1C Polyclonal antibody, PBS Only

CACNA1C Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 21774-1-PBS

Synonyms

Voltage-dependent L-type calcium channel subunit alpha-1C, CCHL1A1, Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle, CACNL1A1, CACN2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CACNA1C Polyclonal antibody (21774-1-AP)

    WB analysis using 21774-1-AP (same clone as 21774-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21774-1-AP (CACNA1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CACNA1C Polyclonal antibody (21774-1-AP)

    IHC staining of mouse brain using 21774-1-AP (same clone as 21774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21774-1-AP (L-VOCC antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CACNA1C Polyclonal antibody (21774-1-AP)

    IHC staining of mouse brain using 21774-1-AP (same clone as 21774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21774-1-AP (L-VOCC antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using CACNA1C Polyclonal antibody (21774-1-AP)

    IHC staining of rat brain using 21774-1-AP (same clone as 21774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21774-1-AP (L-VOCC Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using CACNA1C Polyclonal antibody (21774-1-AP)

    IHC staining of rat brain using 21774-1-AP (same clone as 21774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21774-1-AP (L-VOCC Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CACNA1C Polyclonal antibody (21774-1-AP)

    IHC staining of mouse liver using 21774-1-AP (same clone as 21774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver using 21774-1-AP (L-VOCC antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CACNA1C Polyclonal antibody (21774-1-AP)

    IHC staining of mouse liver using 21774-1-AP (same clone as 21774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver using 21774-1-AP (L-VOCC antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CACNA1C Polyclonal antibody (21774-1-AP)

    IF Staining of mouse brain using 21774-1-AP (same clone as 21774-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L-VOCC antibody (21774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CACNA1C Polyclonal antibody (21774-1-AP)

    IF Staining of mouse brain using 21774-1-AP (same clone as 21774-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L-VOCC antibody (21774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using CACNA1C Polyclonal antibody (21774-1-AP)

    FC experiment of HeLa using 21774-1-AP (same clone as 21774-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human L-VOCC (21774-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21774-1-PBS targets CACNA1C in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CACNA1C fusion protein Ag16455 相同性解析による交差性が予測される生物種
Full Name calcium channel, voltage-dependent, L type, alpha 1C subunit
Calculated molecular weight249 kDa
Observed molecular weight200 kDa
GenBank accession numberBC146846
Gene symbol CACNA1C
Gene ID (NCBI) 775
RRIDAB_2878918
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Calcium voltage-gated channel subunit alpha1 C (CACNA1C, also known as CACH2 and Cav1.2) couples transient activation of inward calcium current to transcriptional regulation and plays an important role in dendritic development, neuronal survival, synaptic plasticity, memory formation, learning, and behavior (PMID: 21248242;  16251435; 20169575; 19047462; 18174367). Genetic variation in CACNA1C has also been associated with depression, schizophrenia, and autism spectrum disorders, as well as changes in brain function and structure in control subjects who have no diagnosable psychiatric illness (PMID: 22705413).