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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using L1CAM Monoclonal antibody (67115-1-Ig)

    WB analysis using 67115-1-Ig (same clone as 67115-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67115-1-Ig (L1CAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig cerebellum tissue using L1CAM Monoclonal antibody (67115-1-Ig)

    WB analysis of pig cerebellum using 67115-1-Ig (same clone as 67115-1-PBS)

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67115-1-Ig (L1CAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using L1CAM Monoclonal antibody (67115-1-Ig)

    IHC staining of human colon cancer using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using L1CAM Monoclonal antibody (67115-1-Ig)

    IHC staining of human colon cancer using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using L1CAM Monoclonal antibody (67115-1-Ig)

    IHC staining of human brain using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using L1CAM Monoclonal antibody (67115-1-Ig)

    IHC staining of human brain using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using L1CAM Monoclonal antibody (67115-1-Ig)

    IHC staining of human colon using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using L1CAM Monoclonal antibody (67115-1-Ig)

    IHC staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using L1CAM Monoclonal antibody (67115-1-Ig)

    IHC staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using L1CAM Monoclonal antibody (67115-1-Ig)

    IF Staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L1CAM antibody (67115-1-Ig, Clone: 3H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using L1CAM Monoclonal antibody (67115-1-Ig)

    IF Staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L1CAM antibody (67115-1-Ig, Clone: 3H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

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L1CAM Monoclonal antibody, PBS Only

L1CAM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67115-1-PBS
Clone No.3H7B9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

L1 cell adhesion molecule, HSAS1, HSAS, CD171, CAML1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67115-1-PBS targets L1CAM in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen L1CAM fusion protein Ag17706 相同性解析による交差性が予測される生物種
Full Name L1 cell adhesion molecule
Calculated molecular weight 1257 aa, 140 kDa
Observed molecular weight 220 kDa
GenBank accession numberBC126229
Gene Symbol L1CAM
Gene ID (NCBI) 3897
RRIDAB_2882419
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP32004
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).