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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

L1CAM Monoclonal antibody, PBS Only

L1CAM Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat, Rabbit

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H7B9

Cat no : 67115-1-PBS

Synonyms

CAML1, CD171, HSAS, HSAS1, L1 cell adhesion molecule, L1CAM, MASA, MIC5, N CAM L1, N CAML1, NCAM L1, S10, SPG1

Validation Data Gallery

Applications:
  • WB analysis using 67115-1-Ig (same clone as 67115-1-PBS)

    WB analysis using 67115-1-Ig (same clone as 67115-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67115-1-Ig (L1CAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • WB analysis of pig cerebellum using 67115-1-Ig (same clone as 67115-1-PBS)

    WB analysis of pig cerebellum using 67115-1-Ig (same clone as 67115-1-PBS)

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67115-1-Ig (L1CAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 67115-1-Ig (same clone as 67115-1-PBS)

    IHC staining of human brain using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 67115-1-Ig (same clone as 67115-1-PBS)

    IHC staining of human brain using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    IHC staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    IHC staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    IF Staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L1CAM antibody (67115-1-Ig, Clone: 3H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    IF Staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L1CAM antibody (67115-1-Ig, Clone: 3H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

67115-1-PBS targets L1CAM in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Pig, Mouse, Rat, Rabbit samples.

Tested Reactivity Human, Pig, Mouse, Rat, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen L1CAM fusion protein Ag17706 相同性解析による交差性が予測される生物種
Full Name L1 cell adhesion molecule
Calculated molecular weight 1257 aa, 140 kDa
Observed molecular weight 220 kDa
GenBank accession numberBC126229
Gene symbol L1CAM
Gene ID (NCBI) 3897
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).