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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

L1CAM Recombinant antibody, PBS Only

L1CAM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240978F1

Cat no : 84034-4-PBS

Synonyms

L1 cell adhesion molecule, HSAS1, HSAS, CD171, CAML1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using L1CAM Recombinant antibody (84034-4-RR)

    WB analysis using 84034-4-RR (same clone as 84034-4-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 84034-4-RR (L1CAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84034-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84034-4-RR

    Affinity and Kinetic Characterization of 84034-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84034-4-RR against Human L1CAM were performed. The affinity constant is 0.255 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84034-4-PBS is L1CAM Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name L1 cell adhesion molecule
Calculated molecular weight 1257 aa, 140 kDa
Observed molecular weight245 kDa
GenBank accession numberBC126229
Gene symbol L1CAM
Gene ID (NCBI) 3897
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).