L1TD1 Polyclonal antibody
L1TD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 21528-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | NCCIT cells |
Positive IHC detected in | human ovary cancer tissue, human ovary tumor tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | NCCIT cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
21528-1-AP targets L1TD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | L1TD1 fusion protein Ag16147 相同性解析による交差性が予測される生物種 |
Full Name | LINE-1 type transposase domain containing 1 |
Calculated molecular weight | 865 aa, 99 kDa |
Observed molecular weight | 100 kDa |
GenBank accession number | BC111559 |
Gene symbol | L1TD1 |
Gene ID (NCBI) | 54596 |
RRID | AB_2878878 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
L1TD1 is an RNA-binding protein highly and specifically expressed in pluripotent cells. It is essential for maintaining the pluripotentency in human embryonic stem cells (hESCs) and has been considered as a marker for undifferentiated hESCs. L1TD1 is also reported to be highly expressed in medulloblastoma cells and its expression in medulloblastoma correlates with poor clinical outcome.
Protocols
Product Specific Protocols | |
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IHC protocol for L1TD1 antibody 21528-1-AP | Download protocol |
IF protocol for L1TD1 antibody 21528-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |