Validation Data Gallery

Filter: AllWBIHC

WB analysis using 15707-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 15707-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 15707-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of SGC-7901 using 15707-1-AP
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 15707-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH Antibody) at dilution of 1:800 incubated at 4 degree celsius over night.
IHC staining of human liver cancer using 15707-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15707-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 15707-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 15707-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

L2HGDH Polyclonal antibody

L2HGDH Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15707-1-AP

Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C14orf160, DURANIN, L2HGDH

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (17)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, rat brain tissue, mouse small intestine tissue, SGC-7901 cells, MCF-7 cells
Positive IHC detected in	human liver cancer tissue, human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 14 publications below
IHC	See 1 publications below

Product Information

15707-1-AP targets L2HGDH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	L2HGDH fusion protein Ag8382 相同性解析による交差性が予測される生物種
Full Name	L-2-hydroxyglutarate dehydrogenase
Calculated molecular weight	463aa,50 kDa; 441aa,49 kDa
Observed molecular weight	45 kDa
GenBank accession number	BC006117
Gene Symbol	L2HGDH
Gene ID (NCBI)	79944
RRID	AB_2133202
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H9P8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

L2HGDH(L-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as duranin, C14orf160 and belongs to the L2HGDH family. The putative L2HGDH is predicted to be targeted to the mitochondria where its mitochondrial targeting sequence is presumably removed(PMID:16005139). Defects in L2HGDH are the cause of L-2-hydroxyglutaric aciduria (L2HGA). It has 2 isoforms produced by alternative splicing with the molecular weight of 50 kDa and 48 kDa. L2HGDH also can be detected as ~45kD due to the 51aa transit peptide cleaved.

Protocols

Product Specific Protocols
WB protocol for L2HGDH antibody 15707-1-AP	Download protocol
IHC protocol for L2HGDH antibody 15707-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Metab Hypoxia Induces Production of L-2-Hydroxyglutarate. Authors - Andrew M Intlekofer KD Validated View Article
human	WB	Cell Metab Hypoxia-Mediated Increases in L-2-hydroxyglutarate Coordinate the Metabolic Response to Reductive Stress. Authors - William M Oldham KD Validated View Article
human	WB	Sci Transl Med 2-Hydroxyglutarate produced by neomorphic IDH mutations suppresses homologous recombination and induces PARP inhibitor sensitivity. Authors - Parker L Sulkowski KD Validated View Article
human	WB	Cancer Discov L-2-Hydroxyglutarate: an epigenetic modifier and putative oncometabolite in renal cancer. Authors - Eun-Hee Shim View Article
human	WB	Cell Chem Biol MYC Regulation of D2HGDH and L2HGDH Influences the Epigenome and Epitranscriptome. Authors - ZhiJun Qiu KO Validated View Article
		Clin Cancer Res Biochemical and Epigenetic Insights into L-2-Hydroxyglutarate, a Potential Therapeutic Target in Renal Cancer. Authors - Sandeep Shelar View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15707-1-AP

WB analysis using 15707-1-AP

WB analysis of mouse small intestine using 15707-1-AP

WB analysis of SGC-7901 using 15707-1-AP

WB analysis of rat brain using 15707-1-AP

IHC staining of human liver cancer using 15707-1-AP

IHC staining of human liver cancer using 15707-1-AP

IHC staining of human gliomas using 15707-1-AP

IHC staining of human gliomas using 15707-1-AP

L2HGDH Polyclonal antibody

L2HGDH Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Hypoxia Induces Production of L-2-Hydroxyglutarate.

Hypoxia-Mediated Increases in L-2-hydroxyglutarate Coordinate the Metabolic Response to Reductive Stress.

2-Hydroxyglutarate produced by neomorphic IDH mutations suppresses homologous recombination and induces PARP inhibitor sensitivity.

L-2-Hydroxyglutarate: an epigenetic modifier and putative oncometabolite in renal cancer.

MYC Regulation of D2HGDH and L2HGDH Influences the Epigenome and Epitranscriptome.

Biochemical and Epigenetic Insights into L-2-Hydroxyglutarate, a Potential Therapeutic Target in Renal Cancer.