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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

L3MBTL3 Polyclonal antibody

L3MBTL3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28112-1-AP

Synonyms

H l(3)mbt like protein 3, KIAA1798, l(3)mbt like 3 (Drosophila), L(3)mbt like protein 3, L3MBTL3, MBT 1, MBT1, RP11 73O6.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using L3MBTL3 Polyclonal antibody (28112-1-AP)

    WB analysis using 28112-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28112-1-AP (L3MBTL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using L3MBTL3 Polyclonal antibody (28112-1-AP)

    IHC staining of mouse cerebellum using 28112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28112-1-AP (L3MBTL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using L3MBTL3 Polyclonal antibody (28112-1-AP)

    IHC staining of mouse cerebellum using 28112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28112-1-AP (L3MBTL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using L3MBTL3 Polyclonal antibody (28112-1-AP)

    IHC staining of mouse cerebellum using 28112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28112-1-AP (L3MBTL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using L3MBTL3 Polyclonal antibody (28112-1-AP)

    IHC staining of mouse cerebellum using 28112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28112-1-AP (L3MBTL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using L3MBTL3 Polyclonal antibody (28112-1-AP)

    IF Staining of U2OS using 28112-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using L3MBTL3 antibody (28112-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28112-1-AP targets L3MBTL3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen L3MBTL3 fusion protein Ag27136 相同性解析による交差性が予測される生物種
Full Name l(3)mbt-like 3 (Drosophila)
Calculated molecular weight 88 aa, 3 kDa
Observed molecular weight88 kDa
GenBank accession numberBC060845
Gene symbol L3MBTL3
Gene ID (NCBI) 84456
RRIDAB_2918148
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

L3MBTL3, also named as MBT1 and KIAA1798, belongs to the Malignant brain tumor (MBT) family. L3MBTL3 can bind to methyl lysine in histone, further affecting the transcription and its abnormal regulation associated with many diseases (PMID: 34373992). Additionally, researches have shown that L3MBTL3 plays a role in meduloblastoma formation and normal hematopoiesis in humans (PMID: 26317848). L3MBTL3 has 2 isoforms with the molecular mass of 86 and 88 kDa.

Protocols

Product Specific Protocols
WB protocol for L3MBTL3 antibody 28112-1-APDownload protocol
IHC protocol for L3MBTL3 antibody 28112-1-APDownload protocol
IF protocol for L3MBTL3 antibody 28112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols