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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LAG-3/CD223 Polyclonal antibody

LAG-3/CD223 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31722-1-AP

Synonyms

CD223, LAG-3, lymphocyte activation gene 3, Ly66, Lag3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAG-3/CD223 Polyclonal antibody (31722-1-AP)

    WB analysis using 31722-1-AP

    Untreated or Con A treated mouse splenocytes were subjected to SDS PAGE followed by western blot with 31722-1-AP (LAG-3/CD223 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inCon A treated mouse splenocytes

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31722-1-AP targets LAG-3/CD223 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name lymphocyte-activation gene 3
Calculated molecular weight57kDa
Observed molecular weight65-75 kDa
GenBank accession numberNM_008479.2
Gene symbol CD223
Gene ID (NCBI) 16768
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)

Protocols

Product Specific Protocols
WB protocol for LAG-3/CD223 antibody 31722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols