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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse LAG-3/CD223 Recombinant antibody, PBS Only (Capture/Detector)

LAG-3/CD223 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241007G10

Cat no : 83941-3-PBS

Synonyms

CD223, LAG-3, Ly66, Lag3, LAG 3

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00927-3

    Sandwich ELISA standard curve of MP00927-3

    Sandwich ELISA standard curve of MP00927-3, Mouse LAG-3/CD223 Recombinant Matched Antibody Pair - PBS only. 83941-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1220. 83941-3-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

  • Sample test of MP00927-3

    Sample test of MP00927-3

    One mouse spleen was cultured for 18 hours in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL of streptomycin sulfate. The cells were stimulated with 5 μg/mL of ConA. An aliquot of the cell culture supernatant was removed, assayed for mouse LAG-3/CD223 and measured 4.12 ng/mL.

  • Cytometric bead array standard curve of MP00927-1

    Cytometric bead array standard curve of MP00927-1

    Cytometric bead array standard curve of MP00927-1, MOUSE LAG-3/CD223 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83941-3-PBS. Detection antibody: 83941-1-PBS. Standard: Eg1220. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00927-2

    Cytometric bead array standard curve of MP00927-2

    Cytometric bead array standard curve of MP00927-2, MOUSE LAG-3/CD223 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83941-3-PBS. Detection antibody: 83941-2-PBS. Standard: Eg1220. Range: 0.313-40 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83941-3-PBS targets LAG-3/CD223 as part of a matched antibody pair:

MP00927-1: 83941-3-PBS capture and 83941-1-PBS detection (validated in Cytometric bead array)

MP00927-2: 83941-3-PBS capture and 83941-2-PBS detection (validated in Cytometric bead array)

MP00927-3: 83941-4-PBS capture and 83941-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name lymphocyte-activation gene 3
Calculated molecular weight57kDa
GenBank accession numberNM_008479.2
Gene symbol CD223
Gene ID (NCBI) 16768
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.