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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody, PBS Only

LAG-3/CD223 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

241294E11

Cat no : 98167-1-PBS

Synonyms

CD223, LAG-3, Ly66, Lag3, LAG 3

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody (98167-1-RR)

    FC experiment of mouse splenocytes using 98167-1-RR (same clone as 98167-1-PBS)

    1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody (98167-1-RR, Clone: 241294E11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98167-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody (98167-1-RR)

    FC experiment of mouse splenocytes using 98167-1-RR (same clone as 98167-1-PBS)

    1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody (98167-1-RR, Clone: 241294E11) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody (98167-1-RR, Clone:241294E11) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98167-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98167-1-RR

    Affinity and Kinetic Characterization of 98167-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98167-1-RR against Mouse LAG-3/CD223 were performed. The affinity constant is 0.636 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98167-1-PBS is LAG-3/CD223 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name lymphocyte-activation gene 3
Calculated molecular weight57kDa
GenBank accession numberNM_008479.2
Gene symbol CD223
Gene ID (NCBI) 16768
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).