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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LAIR1 Polyclonal antibody

LAIR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13297-1-AP

Synonyms

Leukocyte-associated immunoglobulin-like receptor 1, LAIR-1, LAIR 1, hLAIR1, CD305

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAIR1 Polyclonal antibody (13297-1-AP)

    WB analysis using 13297-1-AP

    HeLa cells (LAIR1 negative) and THP-1 cells were subjected to SDS PAGE followed by western blot with 13297-1-AP (LAIR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using LAIR1 Polyclonal antibody (13297-1-AP)

    IHC staining of human ovary cancer using 13297-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13297-1-AP (LAIR1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inTHP-1 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13297-1-AP targets LAIR1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAIR1 fusion protein Ag4021 相同性解析による交差性が予測される生物種
Full Name leukocyte-associated immunoglobulin-like receptor 1
Calculated molecular weight 287 aa, 31 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC027899
Gene symbol LAIR1
Gene ID (NCBI) 3903
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) is a transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs (PMID: 9285412). LAIR1 is expressed on the majority of human PBMCs, including NK, T, B, monocytes, and dendritic cells, as well as the majority of thymocytes (PMID: 10229813). It functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of NK cells, B cells and T cells. LAIR1 is a collagen receptor (PMID: 16754721). Tumor-expressed collagens can modulate immune cell function through the inhibitory collagen receptor LAIR1 (PMID: 21955987).

Protocols

Product Specific Protocols
WB protocol for LAIR1 antibody 13297-1-APDownload protocol
IHC protocol for LAIR1 antibody 13297-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols