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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAMA1 Polyclonal antibody (28697-1-AP)

    WB analysis using 28697-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28697-1-AP (LAMA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of rat colon tissue using LAMA1 Polyclonal antibody (28697-1-AP)

    IF Staining of rat colon using 28697-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using LAMA1 antibody (28697-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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LAMA1 Polyclonal antibody

LAMA1 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 28697-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF-P, ELISA

Synonyms

Laminin 1 subunit alpha, laminin, alpha 1, LAMA, Laminin subunit alpha-1, Laminin subunit alpha 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, U2OS cells
Positive IF-P detected inrat colon tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28697-1-AP targets LAMA1 in WB, IF-P, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAMA1 fusion protein Ag29329 相同性解析による交差性が予測される生物種
Full Name laminin, alpha 1
Calculated molecular weight 337 kDa
Observed molecular weight337 kDa
GenBank accession numberNM_005559
Gene Symbol LAMA1
Gene ID (NCBI) 284217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP25391
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LAMA1 is also known as laminin subunit α1. Laminins are a family of glycoproteins found in the extracellular matrix that comprises the basement membrane. Laminins have a heterotrimeric structure composed of an α, β, and γ chain. Numerous biological processes are known to be directed by them, including cell adhesion, mitogenesis, differentiation, and metastasis, all of which contribute to carcinogenesis. Tissue distribution of LAMA1 occurred mainly in early epithelial development and some adult epithelia. Recent reports have shown that laminin-1 acts as an efficient attachment protein for a large variety of cultured cell types in vitro. (PMID: 36824663)

Protocols

Product Specific Protocols
WB protocol for LAMA1 antibody 28697-1-APDownload protocol
IF protocol for LAMA1 antibody 28697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols