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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAMA2 Polyclonal antibody (31181-1-AP)

    WB analysis using 31181-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31181-1-AP (LAMA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using LAMA2 Polyclonal antibody (31181-1-AP)

    IHC staining of mouse skeletal muscle using 31181-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 31181-1-AP (LAMA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using LAMA2 Polyclonal antibody (31181-1-AP)

    IHC staining of mouse heart using 31181-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31181-1-AP (LAMA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using LAMA2 Polyclonal antibody (31181-1-AP)

    IF Staining of mouse skeletal muscle using 31181-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using LAMA2 antibody (31181-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

LAMA2 Polyclonal antibody

LAMA2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 31181-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

LAMM, Laminin-4 subunit alpha, Laminin-2 subunit alpha, Laminin-12 subunit alpha, Laminin subunit alpha-2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected inmouse skeletal muscle tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31181-1-AP targets LAMA2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAMA2 fusion protein Ag34494 相同性解析による交差性が予測される生物種
Full Name laminin, alpha 2
Calculated molecular weight344 kDa
Observed molecular weight344 kDa
GenBank accession numberNM_000426
Gene Symbol LAMA2
Gene ID (NCBI) 3908
RRIDAB_3669885
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP24043
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The laminin subunit alpha 2 (LAMA2) gene encodes an alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Basal laminae are structural components of the extracellular matrix that influence cell proliferation and differentiation,7 and laminin subunit alpha 2 (LAMA2) is the major component of the basal laminae-α subunit of laminin. (PMID: 30728939)

Protocols

Product Specific Protocols
WB protocol for LAMA2 antibody 31181-1-APDownload protocol
IHC protocol for LAMA2 antibody 31181-1-APDownload protocol
IF protocol for LAMA2 antibody 31181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols